摘要
目的:探讨姜黄素对肝细胞生长因子诱导前列腺癌DU145细胞上皮细胞间质化的影响及可能机制。方法:将DU145细胞分为3组:正常对照组、HGF组、HGF联合姜黄素组。分组干预48小时后,显微镜观察细胞形态变化,划痕试验及Transwell试验检测细胞迁移、侵袭能力,Western blot检测上皮细胞表面标志E-Cadherin和间质细胞表面标志Vimentin的变化,以及HGF/c-Met信号通路分子的表达情况。Real time RT-PCR检测c-Met mRNA的表达变化。结果:分组处理48h,HGF处理组细胞较正常对照组细胞迁移能力显著增强,HGF联合姜黄素处理组细胞迁移能力与HGF处理组相比明显下降;Western blot结果发现,相对于正常对照组,HGF处理组E-cadherin表达水平下降而Vimentin表达水平上升,而姜黄素能明显逆转HGF诱导的EMT相关蛋白表达的改变。进一步研究发现姜黄素能显著下调c-Met mRNA和蛋白水平,进而抑制HGF/c-Met信号下游p-AKT和p-ERK信号以及Snail的表达。结论:姜黄素可逆转HGF诱导的前列腺癌细胞DU145迁移能力,可能与其抑制c-Met的表达和上皮细胞间质化有关。
Objective：To investigate the effect of Curcumin on epithelial-mesenchymal transition in prostate cancer cell line DU145 induced by HGF.Methods：DU145 cells were divided to 3 groups： normal control group,HGF treated group,and HGF ＋ Curcumin treated group.After 48h of treatment,migration of DU145 cells were detected by wound healing and transwell assay.The protein expression of E-cadherin,Vimentin,Snail,c-Met,p-AKT,and p-ERK were determined by Western blot.The mRNA expression of c-Met was determined by Real-time RT-PCR.Results：The migration of DU145 cells induced by HGF was significantly inhibited by Curcumin.Western blot showed that E-cadherin was decreased,whereas Vimentin was increased in HGF treated cells.And these effects were abolished by Curcumin.Meanwhile,Curcumin significantly inhibited c-Met expression,and then inhibited AKT and ERK signaling.Conclusion：Curcumin revised the migration of DU145 cells induced by HGF through inhibiting c-Met expression,and then inhibited the epithelial-mesenchymal transition of DU145 cell.
出处
《现代肿瘤医学》
CAS
2013年第7期1425-1429,共5页
Journal of Modern Oncology
基金
国家自然科学基金资助项目(No.81272311
No.81071712)
