摘要
目的探讨儿茶素对大鼠不同照射时期肺组织转化因子-β1(TGF-β1)、肿瘤坏死因子-α(TNF-α)蛋白的表达,阐述其对急性放射性肺损伤的防治作用及作用机制。方法成年雌性Wistar大鼠40只,随机表法分为4组:A组10只为对照组,腹腔注射生理盐水20 ml/kg/d;B组10只为单纯儿茶素组,腹腔注射儿茶素注射液100 mg/kg/d;C组10只为单纯照射组,全肺单次照射15 Gy+腹腔注射生理盐水20 ml/kg/d;D组10只为照射+儿茶素组,全肺单次照射15 Gy+腹腔注射儿茶素注射液100 mg/kg/d;每组各5只大鼠于照射后2周及6周处死,取肺组织作组织学与免疫组化检测,观察各组大鼠肺组织中TGF-β1、TNF-α蛋白表达的动态变化。结果 C组大鼠2周和6周时肺组织出现明显炎性损伤,D组急性肺炎性改变较C组明显减轻;A组和B组大鼠肺组织免疫组化TGF-β1、TNF-α阳性细胞数相对较低,C组的TGF-β1、TNF-α阳性细胞数较上述两组明显增高(p<0.05),D组阳性细胞数则介于两者之间,也显著低于C组(p<0.05)。结论儿茶素具有抑制TGF-β1、TNF-α蛋白表达,减轻早期放射性肺损伤炎症反应,对急性肺损伤有防治作用,其机制与抑制TGF-β1、TNF-α表达有关。
Objective To observe the effects of catechins on the expression of TGF - β1 and TNF -α on se- rum and lung of rats with radiation - induced lung injury in different radiation time, and discuss the preventative and curative effect and mechanisms of catechins. Methods 40 adauh femal Wistar rats were difined into 4 groups ： those that received neither irriadiation nor catechins （ NT group）, those that received catechins but no irradiation （ CT group）, those that underwent irradiation without catechins （RT group）, those that received both catechins and irradia- tion（ CT/RT group）. Rats were sacrificed at 2 weeks and 6 weeks after irradiation. HE staining and immunohisto- chemical Streptavidin - Peroxidase method were used to examine histologic change of irradiation - induced lung injury and expression of TGF -β1 and TNF -α. Results The RT group rats experienced obvious lung injury at 2 weeks and 6 weeks after irradiation, while CT/RT group rats experienced minor lung injury. NT and CT group exhibited low level of TGF-β1 and TNF -α protein expression. And there was an significantly elevated level of TGF -β1 and TNF-α positive cells in RT group（p 〈 0. 05）. The number of the positive cells in CT/RT group was between NT and CT group and CT/RT group with the difference between CT/RT group and RT group significantly（p 〈 0. 05 ）. Conclu- sion Catechins could inhibit the excretion of TGF-β1 and TNF -α and might play an important role in the preven- tion and curation of radiation - induced lung injury, which might be related to inhibiting the expression of TGF -β1 and TNF -α.
出处
《现代医院》
2013年第6期12-14,共3页
Modern Hospitals
基金
广东省科技计划项目(编号:93053)
