摘要
分别建立了水稻锯齿叶矮缩病毒(RRSV)病株的dsRNA基因组检测法和单头介体褐飞虱带毒的RT-PCR法,并结合生物学接种试验,对介体传毒特性进行了初步分析.结果显示:dsRNA基因组鉴定法可以从0.5 g病株样品中快速检测到RRSV,RT-PCR法可以灵敏地应用于褐飞虱带毒、传毒情况的检测;饲毒后褐飞虱成虫、若虫的带毒率分别为75.0%、68.2%,传毒率分别为50.0%、32.5%,说明褐飞虱种群传播RRSV的能力很强,是高度亲和的群体.
RT-PCR and viral dsRNA identification assays were established to detect Rice ragged stunt virus （RRSV） in Nilaparvata lugens and rice plants, and vector transmission characteristics were analyzed using artificial inoculation. The results showed that vi- ral dsRNA identification assay could detect virus from rice samples as less as 0.5 g, and RT-PCR assay was suitable for detecting RRSV in individual brown planthopper. The viruliferous ratios of nymphs and adults of N. lagens inoculated by RRSV were 68.2% and 75.0%, respectively. 50.0% of nymphs and 32.5% of adults could transmit RRSV after virus acquisition. These results re- vealed that the brown planthopper population had high compatibility to RRSV with strong capacity to transmit the virus.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2013年第3期225-229,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家973计划项目(2010CB126200)
国家自然科学基金项目(30970135)
