摘要
本研究探讨HLA-A、B等位基因多态性与梧州汉族--SEA/αα型α0-地中海贫血患者红细胞参数的相关性。采用聚合酶链反应-直接测序分型法(PCR-SBT),对广西梧州籍汉族57例--SEA/αα型α0-地中海贫血患者进行HLA基因分型,自动血细胞分析仪进行平均红细胞体积(MCV)、血红蛋白(Hb)、平均红细胞血红蛋白含量(MCH)和平均红细胞血红蛋白浓度(MCHC)测定,采用电泳法测定HbA2。应用多分类有序Logistic回归模型进行统计分析。结果表明:HLA-A*33:03、B*15:01或者B*55:02阳性的梧州汉族--SEA/αα型α0-地中海贫血患者Hb、HbA2明显较低,而HLA-B*15:02阳性的患者Hb、HbA2则明显较高(P<0.05)。结论:多个HLA等位基因与梧州汉族--SEA/αα型α0-地中海贫血患者Hb水平可能有关联。研究结果对了解地中海贫血表型与基因型的关系具有一定的参考意义。
This study was purposed to investigate the relationship between HLAA, B allele polymorphisms and red blood cell parameters of patients with sEA/eta subtype of thalassemia in Han ethnic population of Wuzhou city. The HLA genetic polymorphisms were determined by polymerase chain reactionsequencebased typing （PCRSBT） in 57 pa tients with subtype of thalassemia of Han ethnic population in Wuzhou city, Guangxi province, China. Mean corpuscular volume （MCV）, hemoglobin （Hb）, mean corpuscular hemoglobin （MCH） and mean corpuscular hemoglobin concentration （MCHC） were detected by automatic blood analyzer system. HbA2 were detected by electro phoretic method. The statistical analysis was performed by ordinal polytomous logistic regression. The results showed that llb and HbA2 levels were significantly lower in patients positive for HLAA ＊ 33：03, B ＊ 15：01 or B 55：02, and were significantly higher in patients positive for B ＊ 15：02 （ P 〈 0.05 ）. It is concluded that several HLA alleles may be associated with Hb level of SEA/etet subtype of thalassemia of Han ethnic population in Wuzhou city. This result has the value for understanding phenotypegenotype relationships in thalassemia.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2013年第3期688-691,共4页
Journal of Experimental Hematology
基金
梧州市科技计划项目
编号201201063
