摘要
目的探讨单侧纹状体内多靶点注射6-OHDA诱导帕金森病动物模型的制备及其意义。方法利用立体定向技术向纹状体内多靶点(双靶点、三靶点及四靶点)微量注射等量6-OHDA,于注射后2、3、4和6周观察阿朴吗啡诱导的大鼠偏侧旋转行为学改变;采用免疫组化染色检测黑质纹状体TH表达。大鼠经阿朴吗啡诱导偏侧旋转圈数>7r/min(>210r/min),为合格的帕金森病动物模型。结果双靶点诱导组,大鼠成模型率为43.3%(13/30);三靶点诱导组,成模型率为70%(21/30);四靶点诱导组大鼠成模型率为66.7%(20/30)。双靶点和三靶点诱导成模率具有统计学差异(P=0.012);三靶点和四靶点诱导成模率之间无统计学差异(P=0.078)。四靶点组有3只大鼠死亡,其他两组尚未见死亡。6周后黑质纹状体TH凋亡检测二靶点与三靶点之间具有统计学意义,而三靶点与四靶点之间无统计学意义。结论纹状体内三靶点注射不仅能成功诱导帕金森病动物模型,而且能提高动物成模率。
Objective To explore the rat model after 6-OHDA administration by multi-- spots in corpus striatum. Methods The equal amount of 6-OHDA was administrated into corpus striatum by different targets （two spots, three spots and four spots） by means of stereotaxis tech- nique,lateral-rotation by apomorphine and TH immunohistochemistry were observed at 2,3,4 and 6 weeks after 6-OHDA induction. The successful rat model was considered to accord with the follow standardization： the number of lateral--rotation turns should be more than 7r/min （210r/min）, and TH apoptosis by immunostaining was decreased to be 70 % at least in iso--later- al substantia nigra and corpus striatum. Results The successful ratioes of rat models were exhibi- ted as 43.3% （13/30） in two spots lane,70% （21/30） in three spots lane,and 66. 7% （20/30） in four spots lane. The ratio of rat model was significent between two spots and three spots lanes （P =0.012） ,not between three spots and four spots （P--0. 078）. 3 rats died in four spots lane,not occurred in other lanes. TH apoptosis in substantia nigra and corpus striatum were significent be- tween two-- and three spots lanes （P=0. 028）, not occurred between three-- and four spots lanes （P=0. 078）. Conclusion It was an effective measure to induced rat model of PD by three spots administration of 6--OHDA in the corpus striatum.
出处
《立体定向和功能性神经外科杂志》
2013年第2期65-68,共4页
Chinese Journal of Stereotactic and Functional Neurosurgery
基金
国家自然科学青年基金(编号:81100960)
安徽省自然科学青年基金(编号:11040606Q11)
安徽省教育厅课题(编号:KJ2010B381)
关键词
帕金森病
纹状体
动物模型
多靶点
Parkinson＇s disease ~ Corpus striatum
Rat model
Multi-spots
