摘要
建立柑橘、苹果、菠菜、西红柿等水果和蔬菜中乙萘酚的高效液相色谱-串联质谱定量分析检测方法。样品采用乙腈提取,LC-NH2固相萃取柱净化,采用液相色谱-质谱联用技术-电喷雾离子源多反应监测模式检测,外标法定量。结果表明:乙萘酚在50.00～1000.00μg/L范围内线性关系良好(r2≥0.9997),检出限为10μg/kg;在10、20μg/kg和50μg/kg三个添加水平下回收率范围在70.4%～105.5%之间,相对标准偏差(RSD)范围为4.1%～10.6%。
An analytical method based on high performance liquid chromatography-tandem mass spectrometry was developed for the determination of 2-naphthol in fruits and vegetables. Samples were extracted with acetonitrile, and the extract was then cleaned up on an LC-NH2 cartridge. The analytes were determined by LC-MS/MS with electrospray ionization source in multiple reaction monitoring （MRM）. Quantification was conducted by external standard calibration. Good linearity was observed in the range of 50.00 to 1000.00 μg/L （r2 ≥ 0.9997）. The limit of detection of the established method was 10~tg/kg. The recovery rate of 2-naphthol was 70.4%-105.5% at spiked levels of 10, 20 μg/kg and 50 μg/kg in deferent samples, with relative standard deviation of 4.1%-10.6%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第12期213-216,共4页
Food Science
基金
国家质检总局行业标准制定项目(2009B519)
关键词
高效液相色谱-串联质谱
乙萘酚
水果
蔬菜
high performance liquid chromatography-tandem mass spectrometry （LC-MS/MS）： 2-naphthol： fruits
vegetables
