摘要
从重组、突变、迁移与基因流动以及选择等方面探讨了林木病原真菌群体遗传多样性的主要动因 ,从表型观察、致病力和抗药力等特性的人工选择、营养体亲和性测定以及同工酶分析和 DNA分析 (RFLP、RAPD、AFL P、RAMS)等现代分子标记技术几方面讨论了研究林木病原真菌群体分化的主要方法与技术 ,并着重论述了 RAPD技术在林木病原真菌群体分化研究 (如病原真菌的系统分类、亲缘关系与进化、致病性检测与流行性分析 )
The factors affecting the genetic diversity of tree pathogenic population were studied from the aspects of gene recombination,mutation,migration and gene flow.The main research methods and techniques of population differentiation were discussed from the viewpoints of artificial selection of phenotypic observation,virulence and drug resistance,the determination of vegetation affinity and analysis of isozyme,RFLP,RAPD,AFLP,RAMS.The application of RAPD was described in detail.
出处
《林业科学研究》
CSCD
北大核心
2000年第4期423-430,共8页
Forest Research
基金
国家"九五"攻关项目! (95 - 0 4- 0 1 )部分内容