摘要
[目的 ]探讨酚氧化酶 (phenoloxidase,PO)与疟原虫卵囊黑化的关系。 [方法 ]以斯氏按蚊 /约氏疟原虫为模型 ,对 4组斯氏按蚊 (不吸血组、吸正常血组、吸感染血组和硝喹组 )血淋巴进行聚丙烯酰胺凝胶电泳 (PAGE)和凝胶图像分析 ,检测单酚氧化酶 (monophenoloxidase ,MPO)和二酚氧化酶 (diphenoloxidase,DPO)活性。 [结果 ]吸正常血组和不吸血组蚊血淋巴中MPO及o DPO活性无明显差异 ;与吸正常血组或不吸血组相比 ,感染组MPO及o DPO活性无明显变化 ,但用药组d1 0 则显著增加 ,d1 5 显著降低。 [结论 ]斯氏按蚊血淋巴中PO活性变化与约氏疟原虫卵囊黑化在时间上一致。
Objective] To explore the relationship between the hemolymph phenol oxidase and melanization of oocysts. [Methods] Anopheles stephensi Plasmodium yoelii system was used to determine the activity of monophenol oxidase (MPO) and o diphenol oxidase (o DPO) in the hemolymph collected from 4 groups of mosquitoes by polyacrylamide gel electrophoresis (PAGE) followed by density scanning. The 4 groups of mosquitoes were: non blood fed (N), normal blood fed (B), infected blood fed (I) and nitroquine administrated (D), respectively. [Results] No significant difference was found in the activities of MPO and o DPO between groups N and B.The activities of MPO and o DPO were not obviously modified in group I, but were significantly increased on day 10 and decreased on day 15 after blood feeding in the group D as compared with those in the groups N and B. [Conclusion]The alteration in the mosquito hemolymph PO activity coincided at each time point with the melanization of Plasmodium yoelii oocysts.
出处
《中国寄生虫学与寄生虫病杂志》
CSCD
北大核心
2000年第1期11-13,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
全军"九五"科研基金
关键词
约氏疟原虫
斯氏按蚊
血淋巴
酚氧化酶
黑化
Plasmodium yoelii
Anopheles stephensi
hemolymph
phenol oxidase
melanization.
