摘要
采用人工投喂的方法对人工选育的104个"黄海2号"中国对虾(Fenneropenaeus chinensis)家系的5557尾个体[体重(2.1±0.7)g]进行WSSV感染实验,分别选取存活时间最长(342h)和存活时间最短(45h)的48尾个体,定义为抗病组和敏感组。用荧光定量PCR方法检测两组对虾体内WSSV含量,结果表明,抗病组和敏感组对虾体内WSSV含量范围分别为(2.64×104)—(3.13×106)和23.2—1.92×106copies/ngDNA,平均值分别为8.71×105和3.19×105copies/ng DNA,抗病组对虾WSSV含量显著高于敏感组(P<0.05),显示抗病组比敏感组具有更强的抗病性能。对感染测试的所有对虾的死亡情况进行统计分析,结果表明,其死亡曲线不符合数量性状的正态分布特征,提示中国对虾对WSSV的抗性可能由少数几个主基因决定。本研究可为揭示中国对虾抗WSSV性状的基因决定机制提供依据。
A total of 5557 shrimp individuals [（2.1±0.7）g] from 104 families ofFenneropenaeus chinensis ＂Huanghai No.2＂ obtained by selective breeding were artificially infected with WSSV. 48 samples with the longest survival time were defined as the WSSV-resistant group and 48 samples with the shortest were defined as the WSSV-susceptible group. The average survival time of the resistant and the susceptible group was 342h and 45h, with the difference in time of 297h. The WSSV loads in shrimp were quantified by real time PCR. The WSSV loads in the resistant and susceptible group were （2.64×10^4）-（3.13×10^6） and 23.2-1.92×10^6 copies/ng DNA, with the average of 8.71×10^5 and 3.19×10^5 copies/ng DNA, respectively. WSSV loads of the resistant group was significantly higher than the susceptible group （P〈0.05）, which showed higher disease resistance ability of resistant group. The death curve of the shrimp didn＇t accord with the normal distribution of quantitative trait, suggesting that the trait of WSSV resistance may be controlled by several major genes. This study will be helpful to reveal the genetically determined mechanism of WSSV-resistance trait ofF. chinensis.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2013年第3期782-787,共6页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金资助项目,31072206号,31172402号
青岛市关键技术攻关类项目,11-1-1-11-hy号
