摘要
目的 探讨提高脐血移植效果的新方法 ,进一步了解脐血造血干细胞体内生长特性。方法 以经亚致死剂量γ射线照射后的重症联合免疫缺陷小鼠为动物模型 ,进行冻存人脐血的分次移植。利用人粒 单系祖细胞培养及流式细胞仪 ,检测植入小鼠骨髓等器官中人源性细胞。结果与一次移植相比 ,分次移植相同细胞数时 ,小鼠骨髓中人CD45 +、CD34+细胞水平明显提高 ;分次移植细胞数降低到 2 0 %时 ,其造血及部分免疫功能恢复到相近水平。结论 脐血分次移植提高了干细胞的植入率 。
Objective\ To explore a new method for developing the efficiency of human umbilical cord blood (UCB) cells engraftment,and further understand the growth characteristic of hematopoietic stem cells (HSC) in vivo . Methods\ Sublethally irradiated severe combined immunodeficient (SCID) mice were transplanted i.v.with UCB cells which had been cryopreserved at -80℃.The human cells in recipient mice were detected by flow cytometry and CFU\|GM assay. Results\ In contrast to the single transplantation,scheduled engraftment of similar numbers of UCB cells resulted in a proportionally obvious increase in the percentages of CD45 +,CD34 + cells produced in SCID mouse bone marrow (BM).When the donor cells were reduced to 20 percent,an identical reconstitution of both hematopoietic and part of immunologic functions was achieved. Conclusion\ Scheduled engraftment improves the repopulating ability of HSC,which would provide a novel way for clinical cord blood engraftment in adult objects.\;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2000年第4期239-241,共3页
Chinese Journal of Radiological Medicine and Protection
基金
国家自然科学基金!资助项目 (396 0 0 0 77)
关键词
脐血移植
重症联合免疫缺陷
分次移植
实验研究
Umbilical cord blood
\ Hematopoietic stem cells
\ SCID mice
\ Scheduled transplantation
