摘要
目的 研究99Tcm 抗粒细胞单克隆抗体 (BW2 5 0 183)的体内生物学分布及骨髓免疫显像的初步应用。方法 ①利用纸层析法测定不同时相99Tcm BW2 5 0 183的放化纯及其稳定性 ;② 1例正常志愿者注射99Tcm BW2 5 0 183后于不同时相行全身显像 ,并抽取静脉血 ,计算各器官占全身的放射性分布百分数及血液动力学参数 ,同时计数外周血白细胞 ;③利用感兴趣技术对 5例对照者的骨髓免疫显像结果进行定量分析。结果 ①99Tcm BW2 5 0 183标记率高 ,体外稳定 ;②其体内过程属于二室开放系统模型 ,一级动力学类型 ,T1 2α=0 16 3h ,T1 2 β=13 0 6 6h ;③骨髓内放射性分布明显高于肝脏、脾脏 ;④注射99Tcm BW2 5 0 183后外周血白细胞计数无明显降低。结论 99Tcm BW2 5 0 183进入人体后无细胞毒作用 ,骨髓显像图像质量明显优于99Tcm 硫化锝胶体骨髓显像。
Objective To study ①the biological distribution pattern of 99 Tc m BW250/183 after injection ②the primary clinical application of bone marrow radioimmunoimaging Methods ①BW250/183 was labeled following sterile procedure, the labeling rate was analyzed by paper chromatography after being incubated with human serum at 37 ℃ for 15 and 30 min, 1, 2, 4, 6 and 24 h ②One volunteer underwent whole body imaging 15 min, 1, 2, 4, 6 and 24 h after injection of 555 MBq 99 Tc m BW250/183, and 2 mL of blood samples was taken from a cubital vein at every time point The percentage of radioactivity of different organs and the kinetic data of in vivo 99 Tc m BW250/183 was then calculated In all the blood samples the peripheral leukocytes were counted by a standard procedure ③Five control patients underwent whole body and regional imaging 4 h after injection of 555 MBq 99 Tc m BW250/183, the percentage of radioactivity of different organs and the uptake ratios of organ to background were calculated Results The in vivo pharmacokinetics of 99 Tc m BW250/183 belonged to first order reaction, two compartment model T 1/2α and T 1/2β was 0 163 h and 13 066 h, respectively After injection, the distribution of in vivo 99 Tc m BW250/183 was mainly in bone marrow, and the peripheral leukocyte count maintained stable and showed no significant decrease Conclusion 99 Tc m BW250/183 is a safe and ideal bone marrow imaging agent
出处
《中华核医学杂志》
CSCD
北大核心
2000年第4期175-177,共3页
Chinese Journal of Nuclear Medicine
关键词
血液病
放射性核素骨髓显像
抗粒细胞单克隆抗体
Antibodies
monoclonal
Granulocytes
Pharmacokinetics
Bone marrow
Radioimmunodetection
