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ChIP-Seq技术在研究转录因子调控干细胞分化中的应用 被引量：2

Analysis of Transcription Factors Regulated Stem Cell Differentiation by ChIP-Seq
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摘要 研究胚胎发育进程中的转录因子调控及其相关网络,是全面揭示胚胎分化机制需要阐明的首要问题。ChIP-Seq技术凭借其对DNA分子序列及丰度的双重解析能力,已广泛应用于转录因子分子调控机制方面的研究。该文对ChIP-Seq技术和高通量测序技术的发展和更新及其在转录因子调控胚胎发育分化中的研究进展进行了综述,重点论述了ChIP-Seq相关技术在胚胎干细胞和特定肝向分化领域内的最新研究进展。 Study of intricate transcription factor regulatory networks is main issue of illustrating mechanism of embryonic development and differentiation. ChlP-Seq combines chromatin immunoprecipitation （CHIP） with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. ChlP-Seq is widely used to analyze regulatory networks of transcription factors. This paper provides a brief overview of the ChlP-Seq method and application in transcription factors regulation during embyonic development and differentiation, mostly focuses on embryonic stem cell and committed hepatic differentiation.
作者 乌云毕力格 顾婷玉 何志颖 吴侠 李光鹏 李前忠 丁小燕 左永春 王欣
机构地区 内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室 中国科学院上海生命科学研究院生物化学与细胞生物学研究所 第二军医大学细胞生物学教研室 内蒙古大学物理科学与技术学院
出处 《中国细胞生物学学报》 CAS CSCD 北大核心 2013年第6期870-879,共10页 Chinese Journal of Cell Biology
基金 国家自然科学基金(批准号:31060168 31271469 31271474 31271042)资助的课题~~
关键词 染色质免疫共沉淀 转录因子 高通量DNA测序 胚胎 肝脏 ChlP-Seq transcription factor massively parallel DNA sequencing embryo liver
分类号 Q78 [生物学—分子生物学]
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参考文献57

  • 1Messina DN, Glasscock J, Gish W, Lovett M. An ORFeome?based analysis of human transcription factor genes and the con?struction of a microarray to interrogate their expression. Genome Res 2004; 14(10B): 2041-7.
  • 2Muller F, Demeny MA, Tora 1. New problems in RNA poly?merase II transcription initiation: matching the diversity of core promoters with a variety of promoter recognition factors. J Biol Chem 2007; 282(20): 14685-9.
  • 3Im H, Grass JA, Johnson KD, Boyer ME, Wu J, Bresnick EH. Measurement of protein-DNA interaction in vivo by chromatin immunoprecipitation. Methods Mol Biol 2004; 284: 129-46.
  • 4Elnitski L, Jin VX, Farnham PJ, Jones SJ. Locating mammalian transcription factor binding sites: A survey of computational and experimental techniques. Genome Res 2006; 16(12): 1455-64.
  • 5Collas P. The current state of chromatin immunoprecipitation. Mol Biotechnol201O; 45(1): 87-100.
  • 6Orlando Strutt H, Paro R. Analysis of chromatin strncture by in vivo form aldehyde cross-linking. Methods 1997; 11(2): 205-14.
  • 7Lee TI, Johnstone SE, Young RA. Chromatin immunoprecipita?tion and microarray-based analysis of protein location. Nat Pro?toe 2006; 1(2): 729-48.
  • 8Cosseau C, Azzi A, Smith K, Freitag M, Mitta G, Grunau C. Native chromatin immunoprecipitation (N-ChIP) and ChiP-Seq of Schistosoma mansoni. Critical experimental parameters. Mol Biochem Parasitol 2009; 166(1): 70-6.
  • 9Sun JM, Chen HY, Davie JR. Differential distribution of un modi?fied and phosphorylated histone deacetylase 2 in chromatin. J Biol Chem 2007; 282(45): 33227-36.
  • 10李敏俐,王薇,陆祖宏.ChIP技术及其在基因组水平上分析DNA与蛋白质相互作用[J].遗传,2010,32(3):219-228. 被引量：21

二级参考文献123

  • 1Harris T D, Buzby P R, Babcock H, et al. Single-molecule DNA sequencing of a viral genome. Science, 2008, 320:106-109.
  • 2Harris T D, Buzby P R, Jarosz M, et al. Optical train and method for TIRF single molecule detection and analysis. US patent application 20070070349, 2007.
  • 3Hardin S, Gao X, Briggs J, et al. Methods for real-time single molecule sequence determination. US patent 7329492, 2008.
  • 4Eid J, Fehr A, Gray J. et al. Real-Time DNA sequencing from single polymerase molecules. Science, 2009, 323:133-138.
  • 5Levene M J, Korlach J, Turner S W, et al. Zero-mode waveguides for single-molecule analysis at high concentrations. Science, 2003, 299: 682-686.
  • 6Korlach J, Marks P J, Cicero R L, et al. Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures. Proc Nail Acad Sci USA, 2008, 105:1176-1181.
  • 7Array based sequencing-by-synthesis, http: Hwww.mobious.com.
  • 8Densham D H. Nucleic acid sequence analysis. EU Patent Application EP 1229133, 2002.
  • 9Driscoll R J, Youngquist M G, Baldeschwieler J D. Atomic-scale imaging of DNA using scanning tunnelling microscopy. Nature, 1990, 346: 294-296.
  • 10Ikai A. TM and AFM of biolorganic molecules and structures. Sur Sci Rep, 1990, 26:263-332.

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中国细胞生物学学报
2013年 第6期

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