摘要
运用计算机及实验的方法，在粟酒裂殖酵母（Ｓｃｈｉｚｏｓａｃｃｈａｒｏｍｙｃｅｓ ｐｏｍｂｅ）中发现和鉴定了一个新的、结构特殊的ｓｎｏＲＮＡ──Ｚ１５．该ｓｎｏＲＮＡ由一个独立转录的基因编码，位于酵母２号染色体上Ｚ１５长达为９２个核苷酸，属于反义ｓｎｏＲＮＡ家族．它除了具有典型的ｂｏｘＣ／Ｄ结构元素和末端配对区外、还含有分别与酿酒酵母Ｚ１５和Ｚ２相同的两个反义序列，反映了粟酒裂殖酵母川Ｚ１５ｓｏｎＲＮＡ基因可能通过２种ｓｎｏＲＮＡ基因的重组而来的．推测Ｚ１５除了指导２５Ｓ ｒＲＮＡ Ａ８４９和Ａ９０８的２’－氧－核糖的甲基化修饰外，还可能在ｒＲＮＡ构象形成中起作用．粟酒裂殖酵母Ｚ１５的发现．从一个新的角度揭示了ｓｎｏＲＮＡ基因结构和功能的复杂性，并为研究这类ｓｎｏＲＮＡ基因的起源和进化提供了重要线索．
A novel small nueleolar RNA, termed Z15, was identified and characterized by the analysis of computer and experiments. Z15 snoRNA, encoded by an independent-transcribed gene on chromosome Ⅱ of S .pombe,is 92nt in length and belongs to the antisense sonRNA family in possessing boxC/D motif and terminal stem-forming sequences. In particularly, the Z15 snoRNA has two antisense sequences identical to that of Saccharomyces cerevisiae Z15 and Z2 snoRNA respectively, this mosaic structure implies that S. pombe Z15 originated probably from two different snoRNAs by gene recombination. According to the structural analysis, S. pombe Z15 is Presumed not only to function as a methylation guide for 25S rRNA at position A849 an fi A908, but also as a RNA chaperon for rRNA conformation.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第2期56-60,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金重点资助项目!(39730300)
国家教育部重点基金资助项目
关键词
粟酒裂殖酵母
RNA制备
甲基化
Z15
snoRNA
Schizosaccharomyces,pombe
rRNA
methylation
