摘要
目的探讨S期激酶相关蛋白2(Skp2)及磷酸酶和张力蛋白基因(PTEN)在不同子宫内膜组织中的表达及其与子宫内膜癌发生、发展的关系。方法选取中国医科大学附属盛京医院2008-2010年手术切除的59例子宫内膜腺癌标本。患者年龄36～70岁,平均54岁。另选取30例不典型增生子宫内膜和30例增生期子宫内膜标本,应用免疫组织化学SP法和免疫印迹法(western blotting)检测Skp2和PTEN蛋白的表达水平,分析其表达水平与临床病理参数的关系以及二者的相互关系。结果 Skp2在子宫内膜癌中表达高于不典型增生和正常增生期内膜,差异有统计学意义(P<0.05),在晚期子宫内膜癌、低分化组和有淋巴结转移组较早期、高中分化组和无淋巴结转移组表达增高,差异有统计学意义(P<0.05)。PTEN在子宫内膜癌中的表达低于不典型增生和正常增生期内膜,差异有统计学意义(P<0.05),在晚期子宫内膜癌和低分化组表达低于早期和高中分化组,差异有统计学意义(P<0.05)。在子宫内膜癌中Skp2表达增高,PTEN表达下降,二者呈负相关。结论 Skp2和PTEN可能参与子宫内膜癌的发生发展过程。
Objective To detect the expression of Skp2 and PTEN in different endometrial tissues and explore the relationship between Skp2, PTEN and endometrial carcinoma. Methods We select the surgical removal of the 59 cases of endometrial adenocarcinoma specimens of 2008-2010 ,in Shengjing Hospital of the China Medical University. Patients aged 36 to 70 years, with an average age of 54 years, detect the expression of Skp2 and PTEN by imnmnohistochemistry method and western blotting,then analyze their association with the clinicalpatho-parameter. Results There is a higher expression of Skp2 in endometrial carcinoma than in atypical hyperplasia endometrium and proliferative phase endometri- um（ P 〈 0. 05 ）. Skp2 has a higher expression in late stage, low-differentiation and with lymph nodes metastasis groups than early stage, high-moderated-differentiation and non lymph nodes metastasis groups （ P 〈 0. 05 ）. There is a lower expression of PTEN in endometrial carcinoma than atypical hyperplasia endometrium and proliferative phase endomemtrium （ P 〈 0. 05 ）. PTEN has a lower expression in late stage,low-differentiation groups than early stage, high-moderated-differentiation groups （ P 〈 0. 05 ）. Skp2 has a high expression in endometrial carcinoma, and PTEN has a low expression, there is a negative correlation between them. Conclusion Skp2 and PTEN may be involved in the process of endometrial carcinoma.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2013年第6期468-472,共5页
Chinese Journal of Practical Gynecology and Obstetrics
基金
沈阳市科学技术计划应用基础研究项目(F12-193-9-12)
