摘要
目的:观察丹参酮Ⅱ-A磺酸钠对房颤大鼠心房肌细胞电重构及细胞膜钾离子通道的影响。方法:制备房颤动物模型,荧光定量PCR技术测定心房肌细胞Kv1.5钾通道基因表达,HE染色观察心房肌细胞超微结构变化。结果:丹参酮Ⅱ-A磺酸钠、胺碘酮能明显改善房颤大鼠心房肌细胞钾通道Kv1.5基因表达减低水平(P<0.05)及心房肌细胞组织超微结构。结论:丹参酮Ⅱ-A磺酸钠能够通过改善房颤大鼠钾通道Kv1.5基因表达下调所引发的心房电重构,从而预防和治疗房颤。
Objective： To observed the tanshinone Ⅱ-A sulfonate atrial fibrillation rat atrial myocytes electrical remodeling and cell membrane potassium ion channels. Methods： Preparation of atrial fibrillation animal models, quantitative PCR determination of atrial myocytes Kvl.5 potassium channel gene expression, HE staining atrial myocytes ultrastructural changes. Results： Tanshinone Ⅱ-A sulfonate, amiodarone significantly improved atrial fibrillation rat atrial myocytes potassium channel Kvl.5 gene expression level reduction （P〈0.05） and atrial muscle cell ultrastructure. Conclusion： Tanshinone Ⅱ-A sulfonate by improving atrial fibrillation rats potassium channel Kvl.5 gene expression caused by atrial electrical remodeling cut to the prevention and treatment of atrial fibrillation.
出处
《中医临床研究》
2013年第9期10-13,共4页
Clinical Journal Of Chinese Medicine
基金
贵州省卫生厅项目
贵阳市科技局项目:筑科合同[2011103]30号
贵州省科技厅
贵阳中医学院联合基金[黔科合中药字(2010)LKZ7012]
关键词
丹参酮Ⅱ-A磺酸钠
房颤
心房电重构
Kv1.5钾通道
Tanshinone Ⅱ-A sulfonate
Atrial fibrillation
Atrial electrical remodeling
Kvl.5 potassium channel
