摘要
目的:分离提纯原人参二醇组皂苷(PPD),建立一种新的PPD皂苷的分离方法,为其分离提供理论依据.方法:本研究通过静态解吸试验,探索不同乙醇浓度对D101C大孔吸附树脂静态解吸PPD和PPT皂苷的影响,再结合丙酮沉淀法提纯PPD皂苷.结果:HPLC分析表明,将浓度为15 mg/mL的人参根总皂苷溶液用D101C大孔吸附树脂于室温下吸附12 h,再用35%乙醇溶液进行静态解吸,PPD和PPT皂苷的比值达到最大值,由解吸前的3.43提高到19.59.经80%乙醇溶液解吸后,用70%乙醇溶解,加入适量丙酮得PPD皂苷,其纯度由原料的61.32%提高到96.29%.结论:该方法操作简单,所得产品纯度高,是分离提纯PPD皂苷的简便而有效的方法.
Objective ： To provide a theoretical basis about separation of protopanaxadiol type saponins （ PPD）, they were separated and purified, and a new method was founded. Method ： The influences of ethanol of different concentration on static desorption of PPD and PPT saponins with DIO1C macroporous adsorption resin was studied by static desorption experiments. Then PPD saponins were further purified by the method of acetone precipitation. Result：The analysis of HPLC showed that the solution of ginseng root saponins （ 15 mg · mL-1 ） was adsorbed by DIO1C macroporous adsorption resin at room temperature for 12 h. Then statically desorbed with 35% ethanol, the ratio of PPD and PPT saponins increased from 3.43 to 19. 59 and achieved its maximum. PPD saponins were achieved after desorbed with 80% ethanol, dissolved with 70% ethanol and added appropriate acetone, the purity increased from 61.32% of the raw material to 96.29%. Conclusion：This method by which product is high purity is operation-simple. Itg a convenient and effective way to separate and purify PPD saponins.
出处
《吉林化工学院学报》
CAS
2013年第5期54-58,共5页
Journal of Jilin Institute of Chemical Technology
基金
吉林化工学院博士科研启动基金(20080414)
吉林市科技局项目(201222316)
关键词
D101C大孔吸附树脂
静态解吸
分离提纯
原人参二醇组皂苷
原人参三醇组皂苷
ginseng root saponins
D101C macroporous adsorption resin
static desorption
separation and purification
protopanaxadiol type saponins
protopanaxatriol type saponins
