摘要
目的:对卵巢子宫内膜异位囊肿患者血清中的β-actin进行检测,试图为EMs寻找新的标志物作为检测对象应用于临床。方法:采用酶联免疫吸附(ELISA)的方法检测子宫内膜异位症(EMT)和对照组血清中β-actin的表达,再查阅以上各组标本的病历,找到与之相关的实验室检查指标。结果:对40例标本进行3次重复实验,EMT血清中β-actin的平均含量高于卵巢恶性肿瘤组(P<0.05)。经查阅病历后将各组患者血清中CA125的检测结果进行统计学分析,发现CA125在卵巢子宫内膜异位囊肿中较卵巢恶性肿瘤低表达。结论:β-actin在鉴别卵巢子宫内膜异位症与卵巢恶性肿瘤中有一定的参考价值,且β-actin与CAl25联合检测可以提高其特异性及敏感性。
Objective：To detect β -actin in serum of patients with ovarian endometriosis cyst, and try to search new biomarkers as detection object for endometriosis in clinic. Methods： ELISA was used to detect the expression levels of serum β - actin in endometriosis group and control group, the related laboratory indexes were found by consulting the medical records. Results：Three experiments were conducted repeatedly among 40 specimens, the average content of serum β - actin in endometriosis group was statistically significantly higher than that in malignant ovarian cancer group （P 〈 0. 05 ）. After consulting medical records, the expression level of serum CA125 in ovarian endometriosis cyst was lower than that in malignant ovarian cancer. Conclusion： β - actin has a certain reference value for differentiating ovarian endometriosis from malignant ovarian cancer, and joint detection of β - actin and CA125 can improve the specificity and sensitivity.
出处
《中国妇幼保健》
CAS
北大核心
2013年第17期2700-2702,共3页
Maternal and Child Health Care of China
