摘要
目的:观察附子理中汤对脾阳虚大鼠水通道蛋白4(AQP4)的影响,探讨附子理中汤止泻作用的机制。方法:Wistar大鼠100只随机分为对照组、模型组、附子理中汤低、中、高剂量组5组,每组20只。在模型组基础上,低剂量组按10 g.kg-1ig,中剂量组按20 g.kg-1ig,高剂量组按40 g.kg-1ig,每天1次,连续4周。取结肠标本,酶联免疫法(ELISA)法检测AQP4含量,RT-PCR法检测AQP4 mRNA表达。结果:和对照组比较,模型组的AQP4含量(13.25±4.22)ng.L-1.AQP4mRNA相对表达量(0.38±0.11)均降低(P<0.01或P<0.05)。和模型组比较,附子理中汤中剂量组的AQP4含量(23.84±5.68)ng.L-1,AQP4 mRNA相对表达量(0.54±0.26)均升高(P<0.05)。和模型组比较,高剂量组的AQP4含量(28.98±6.12)ng.L-1,AQP4 mRNA相对表达量(0.62±0.32)均显著升高(P<0.01)。结论:附子理中汤能恢复脾阳虚大鼠AQP4含量和AQP4 mRNA正常表达,以高剂量的恢复作用较明显,附子理中汤可能通过上调AQP4表达,促进胃肠道黏膜对水液的重吸收而起到止泻的作用。
Objective： To observe effect of Fuzi Lizhong decoction on aquaporin 4（AQP4） in rats with spleen Yang deficiency syndrome and discuss its antidiarrheal mechanism.Method： One hundred Wistar rats were randomly divided into control group,model group,low-dose group,middle-dose group and high-dose group,20 rats each group.low-dose group,middle-dose group and high-dose group were ig given with Fuzi Lizhong decoction according to 10,20,40 g.kg-1 based on model group,once a day for four weeks.colon was collected as sample to examine content of AQP4 by ELISA method and expression of AQP4 mRNA by RT-PCR method.Result： Compared with control group,in model group both content of AQP4（13.25 ± 4.22） ng.L-1 and expression of AQP4 mRNA（0.38 ± 0.11） reduced（P ＆lt; 0.01 or P ＆lt; 0.05）.Compared with model group,in middle-dose group both content of AQP4（23.84 ± 5.68） ng.L-1and expression of AQP4 mRNA（0.54 ± 0.26） increased（P ＆lt;0.05）.Compared with model group,in high-dose group both content of AQP4（28.98 ±6.12） ng.L-1and expression of AQP4 mRNA（0.62 ± 0.32） increased significantly（P ＆lt; 0.01）.Conclusion： Fuzi Lizhong decoction could recover content of AQP4 and expression of AQP4 mRNA,and high dose had a better effect.Fuzi Lizhong decoction could up-regulate expression of AQP4 mRNA and promote gastrointestinal reabsorption of water,which perhaps are one of its antidiarrheal mechanisms.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期222-225,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
附子理中汤
脾阳虚证
水通道蛋白4
Fuzi Lizhong decoction
spleen Yang deficiency syndrome
aquaporin-4
