摘要
目的:建立益母草、茺蔚子的体外抗氧化活性测定方法,对比益母草、茺蔚子抗氧化活性,为指导益母草和茺蔚子的临床合理用药提供支持。方法:益母草、茺蔚子充分粉碎,室温下用75%乙醇超声30 min制备益母草、茺蔚子提取液,用二苯代苦味酰基自由基(DPPH.)法测定二者的抗氧化活性,并跟维生素E比较,评价益母草、茺蔚子的体外抗氧化能力。结果:益母草、茺蔚子以及维生素E溶液对自由基清除率的半数有效浓度(EC50)分别为2.70,1.28,5.89 g.L-1,表明其抗氧化活性:茺蔚子提取液>益母草提取液>维生素E溶液;重复性考察结果表明实验结果重复性较好(益母草RSD 3.34%,茺蔚子RSD 2.25%)。结论:益母草和茺蔚子在体外都具有较强抗氧化活性,且茺蔚子抗氧化活性强于益母草。
Objective： To establish a method to assay antioxidant activity in vitro of Leonuri Herba and Leonuri Fructus,as a guide for clinical rational use of Leonuri Herba and Leonuri Fructus.Method： 75% ethanol extracting solutions of Leonuri Herba and Leonuri Fructus were prepared with ultraphonic for 30 min at 25 ℃,and their antioxidant capacity were evaluated with 1,1-Diphenyl-2-picrylhydrazyl radical-2,2-Diphenyl-1-（2,4,6trinitrophenyl） hydrazyl（DPPH.） spectrometry,compared with vitamin E.Result： The extracts of Leonuri Herba and Leonuri Fructus both had good scavenging effects on DPPH..IC50 of Leonuri Herba extract,Leonuri Fructus extract and vitamin E solution were 2.70,1.28,5.89 g.L-1,respectively.In other words the antioxidant capability was： Leonuri Fructus extract ＆gt; Leonuri Herba extract ＆gt; vitamin E.The repeatability inspection were： Leonuri Herba,RSD 3.34%;Leonuri Fructus,RSD 2.25%.Conclusion： Leonuri Fructus has a stonger antioxidant than Leonuri Herba in vitro.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期179-181,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金面上项目(81173474)
郑州市科技攻关计划(112PPTSF319-4)
河南中医学院博士科研基金(BSJJ2009-09)
