摘要
目的:优选大叶紫珠总黄酮的提取工艺并评价其抗炎、镇痛、止血作用。方法:以总黄酮提取率为指标,在单因素试验基础上,采用正交试验考察乙醇体积分数、提取次数、提取时间及料液比对大叶紫珠总黄酮提取工艺的影响;通过二甲苯致耳肿胀法、醋酸扭体法、毛细玻管法依次评价大叶紫珠总黄酮的抗炎、镇痛、止血作用。结果:最佳提取工艺为加20倍量90%乙醇于80℃回流2次,每次2 h,总黄酮提取率63.1 g.kg-1。大叶紫珠总黄酮提取物经SP825型大孔吸附树脂和聚酰胺树脂纯化后,总黄酮纯度达53.3%。动物试验表明中、高剂量的大叶紫珠总黄酮表现出明显的抗炎、镇痛及止血作用,低剂量时仅表现出镇痛作用。结论:优选的工艺能达到富集有效成分的目的,大叶紫珠总黄酮具有抗炎镇痛止血作用,且呈剂量相关性。
Objective： To optimize extraction process of total flavonoids from Callicarpa macrophylla（TFCM） and estimate its anti-inflammatory,antalgic and hemostatic activities.Method： With yield of total flavonoids as index,on the basis of single factor test,effect of the concentration of ethanol,liquid-solid ratio,extraction times and time on extraction technology of TFCM;Anti-inflammatory,antalgic and hemostatic activities of TFCM were evaluated by xylene-induced ear edema,acetic acid-induced writhing,glass capillary tube method in mice,respectively.Result： Optimal extraction parameters of TFCM were ethanol concentration 90%,ratio of solid to liquid 1∶20,extraction temperature 80 ℃,extracted 2 times with 2 h per time.Under these conditions,yield of TFCM was 63.1 g.kg-1.After purified by macroporous resin and polyamide resin,purity of TFCM was up to 53.3%.TFCM displayed potent anti-inflammatory,antinociceptive activity and significantly shortened the blood coagulation time in mice at the dose of 200 and 400 mg.kg-1,but TFCM merely exhibited antalgic activity at the dose of 100 mg.kg-1.Conclusion： Optimized process could achieve purpose of enrichment of active ingredients,TFCM possessed significant anti-inflammatory,antalgic and hemostatic activities with dose-related.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期8-11,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"十一五"科技支撑项目(2006BAI06A19)
关键词
大叶紫珠
总黄酮
提取
纯化
抗炎
镇痛
止血
Callicarpa macrophylla
total flavonoids
extraction
purification
anti-inflammation
analgesia
hemostasis
