摘要
目的探讨冬眠合剂对大鼠急性脑梗死早期脑组织中基质金属蛋白激酶-9(Matrix metalloproteinase-9,MMP-9)和血小板活化因子(Platelet-Activating Factor,PAF)表达的变化及可能的作用机制。方法成年雄性SD大鼠126只随机分成对照组、模型组及亚冬眠组(造模后肌注冬眠合剂Ⅱ号)3组,均于缺血后4h、12h、24h进行神经功能评分,随之进行HE染色观察大鼠脑梗死的大体形态,免疫组化Sp法检测PAF、RT-PCR技术检测MMP-9的表达。结果与模型组相比,亚冬眠组MMP-9及PAF的含量减少(P<0.05),神经功能缺损评分降低(P<0.05).结论冬眠合剂对大鼠急性脑梗死损伤早期有脑保护作用。
Objective To investigate the expression change and the possible mechanism of matrix metalloproteinase-9（MMP-9）and Platelet-Activating Factor（PAF）in rat brain tissue at the background of lytic cocktail on the early acute cerebral infarction injury.Method 126 adult male SD rats were randomly divided into three groups：control group,model group and sub-hibernation group（giving lytic cocktailⅡintramuscularly after the modeling）.The neurological scores of each group were evaluated at 4 hours,12 hours,24 hours after ischemia.Then,the general organic shape of the ischemic rats brain tissue was detected by HE staining,the expression of PAF was detected by immunohistochemical Sp and the expression of MMP-9 was detected by the RT-PCR technical.Result Compared with model group,MMP-9 and PAF content decreased in the sub-hibernation group（P〈0.05）,neurological deficit scores were reduced（P〈0.05）.Conclusion Lytic cocktail has the early cerebral protective effect on acute cerebral infarction injury.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2013年第5期450-453,共4页
Journal of Apoplexy and Nervous Diseases
