摘要
目的:探讨磁共振影像技术评价大鼠肌肉组织坏死模型的实验方法,并用病理金标准验证磁共振扫描结果。方法:向6只大鼠左后肢注射0.2 ml无水乙醇,制成骨骼肌坏死模型,分别于造模后0、2、4、8、12、14、16 h进行磁共振扫描,分析图像并得出最佳扫描序列及最佳扫描时间。另取20只模型鼠在最佳扫描条件下行磁共振扫描,扫描后处死行病理检查。对病变区伊文氏蓝染色大体病理图像及T2WI fs图像进行形态比较,并对两者面积比进行分析。结果:T2WI fs时病变范围清晰且排除周围脂肪信号的干扰,为最佳监测序列,12 h为最佳扫描时间。坏死骨骼肌面积在磁共振影像和伊文氏蓝染色大体病理影像上无明显差异。磁共振影像上左后肢坏死骨骼肌面积占可视面积的(7.95±0.18)%,伊文氏蓝染色大体病理影像上左后肢坏死骨骼肌面积占可视面积的(7.91±0.15)%。结论:磁共振影像可作为大鼠骨骼肌坏死模型的评价方法。
Objective：To investigate an experimental method of using magnetic resonance imaging （MRI） techniques to evaluate rat muscle tissue necrosis model. Methods：Firstly,0.2 ml anhydrous ethanol was injected into the left hind limbs of 6 rats to make a skeletal muscle necrosis model. MRI scans were performed after 0,2,4,8,12, 14 h and 16 h respectively to analyze imagines and to find optimal scanning sequence and scanning time. Results： MRI scanning resulted from T2WI fs and 12 h showed clear pathological changing area and excluded the signal interference from peripheral fat,which proved T2WI fs is the optimized scanning sequence and 12 h is the optimized scanning time. Another 20 model rats were used for MRI scans with the optimal scanning sequence and time, then executed after MRI scans for pathological examination. There was no apparent size difference between Evans blue dye pathology images and MRI images of rat skeletal muscle necrosis area. The skeletal muscle necrosis area was （7.95 ± 0.18）% of the viewing area of the left hind in MRI images,and was （7.91 ± 0.15）% in/he Evans blue dye pathology images. Conclusion：MRI scans can be used for evaluating skeletal muscle necrosis.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第5期702-706,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省自然科学基金(BK2010594)
关键词
肌肉坏死
磁共振成像
病理检查
muscle necrosis
magnetic resonance imaging
pathologic examination
