摘要
目的:探讨川芎嗪(Tetramethylpyrazine,TMP)对PC12细胞氧糖剥夺/复氧复糖损伤后细胞内抗超氧阴离子能力和过氧化氢(H2O2)的影响。方法:建立PC12细胞氧糖剥夺(Oxygen-Glucose Deprivation,OGD)模型,复氧复糖给予不同浓度(0.01、0.02、0.04、0.08、0.16、0.32、0.64、1.28、2.56、5.12、10.24μmol/L)的川芎嗪,培养24 h后行MTT和LDH的检测。采用四甲基偶氮唑(Methyl Thiazolyl Tetrazolium,MTT)比色检测细胞生存率,并检测细胞内抗超氧阴离子能力和过氧化氢。结果:氧糖剥夺可明显降低PC12细胞活性(55.05%),而川芎嗪可以显著提高PC12细胞活性,其作用呈现一定的剂量-效应相关性。复氧复糖后给予0.16μmol/L川芎嗪可以提高PC12细胞的活性到75.38%。氧糖剥夺/复氧复糖后6 h、24 h,TMP组(川芎嗪组)抗超氧阴离子能力明显高于OGD组(模型组)(P<0.05),而H2O2的含量TMP组明显低于OGD组(P<0.05)。结论:川芎嗪可能提高抗超氧阴离子能力,减少H2O2的产生,从而减轻PC12细胞氧糖剥夺/复氧复糖损伤,具有较好的治疗作用。
Objective： To investigate effects of tetramethylpyrazine（TMP） on superoxide anion and hydrogen peroxide in oxygenglucose deprivation（OGD）/restoration-induced PC12 cells.Methods： The cells in OGD group were exposed to OGD for 4 h followed by re-oxygenation for 24h.The cells in TMP group were treated with various concentrations of TMP（0.01,0.02,0.04,0.08,0.16,0.32,0.64,1.28,2.56,5.12,10.24 μmol/L） after ODG for 4 h.The cell viability was determined by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） assay.The intracellular superoxide anion and hydrogen peroxide（H2O2） were detected at 6 h,24 h after re-oxygenation in the PC12 cells.Results： Compared with that in the normal PC12 cells,cell viability in OGD group declined to 55.05%（P0.01）.Compared with PC12 cells exposed to OGD,cell viability in TMP group markedly increased in a dose-dependent manner.The viability achieve up to75.38% at TMP concentration of 0.16 μmol/L.Compared with that in the normal PC12 cells,the product of H2O2increased and superoxide anion decreased in OGD group at 6 h,24 h after re-oxygenation（P0.05）,but 0.16 μmol/L of TMP could inhibit those changes.Conclusion： The protective effects of tetramethylpyrazine against PC12 exposed to OGD might be related to reducing the product of H2O2,increasing the ability against superoxide anion.
出处
《现代生物医学进展》
CAS
2013年第16期3037-3040,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81001566)
关键词
川芎嗪
PC12细胞
超氧阴离子
过氧化氢
氧糖剥夺
复氧复糖
Oxygen-glucose deprivation（OGD）
PC12 cells
Tetrameylpyramine（TMP）
Superoxide anion
Hydrogen peroxide（H2O2）
