摘要
用异硫氰酸苯酯柱前衍生高效液相色谱法测定了淡水养殖珍珠中角壳蛋白的氨基酸组成 ,对影响木瓜酶水解珍珠角壳蛋白的主要因素如木瓜酶用量、蛋白质质量浓度、反应时间、酸度和温度等进行了研究和优化 。
The contents of amino acids in protein of pearls were determined by reverse-phase high-performance liquid chromatography after precolumn derivatization with phenylisothiocyanate. The effect of such factors as dosage of papain, concentration of protein, time of reaction, acidity, temperature on hydrolytic ratio of protein in pearls by papain was studied. A simple and no-pollution technique for hydrolyzing protein in pearls by papain to obtain amino acids and peptides was developed in order to provide a basis for the effective use of pearl resources in our country.
出处
《江苏石油化工学院学报》
2000年第3期33-35,共3页
Journal of Jiangsu Institute of Petrochemical Technology
