摘要
目的探讨Raf激酶抑制蛋白(RKIP)、NF-κBp65和血管内皮生长因子(VEGF)-C在上皮性卵巢肿瘤发生、发展中的作用和可能的分子机制。方法以免疫组织化学SP法检测手术切除卵巢标本中正常卵巢组织(20例)、卵巢良性上皮性肿瘤(18例)、交界性肿瘤(17例)、上皮性卵巢癌组织(32例)中RKIP、NF-κBp65和VEGF-C蛋白的表达,分析各组间这三者表达的变化与临床、病理因素之间的关系,以及三者间的相关性。结果 (1)与正常卵巢组织和卵巢良性上皮性肿瘤组织比较,上皮性卵巢癌组织中RKIP的表达阳性率(28.13%vs90.00%,p<0.01;28.13%vs55.56%,P<0.05)显著降低;NF-κBp65蛋白的表达阳性率(81.25%vs15.00%P<0.01;81.25%vs50.00%,P<0.05)显著增高;VEGF-C的表达阳性率(90.63%vs40.00%,P<0.01;90.63%vs44.44%,P<0.01)显著增高。(2)上皮性卵巢癌中RKIP、NF-κBp65的表达与患者年龄、组织学类型及病理分级无相关性(P均>0.05),与临床分期有相关性(P<0.05);VEGF-C的表达与患者年龄、组织学类型、病理分级及临床分期无关(P均>0.05)。(3)上皮性卵巢癌组织中RKIP与NF-κBp65的表达呈负相关(r=-0.4338,P=0.0131)。结论 RKIP、NF-κBp65和VEGF-C可能参与上皮性卵巢癌的发生与发展,RKIP可能通过调控NF-κB信号通路在上皮性卵巢癌的侵袭与转移中发挥作用。
Objective To explore the function of Raf kinase inhibitory protein （RKIP）, NF-KBp65 and VEGF-C protein in epithelial ovarian tumors occurrence and development as well as the possible molecular mechanisms. Methods We detected the expression of RKIP, NF-KBp65 and VEGF-C protein in normal ovarian tissues（20 cases）, Ovarian benign epithelial tumors（ 18 cases）, borderline ovarian tumors（ 17 cases） and epithelial ovarian cancers（32 cases） by SP immunohistochemical assay, and analyzed the expression changes of RKIP, NF- KBp65 and VEGF-C between different groups, as well as the relationship between RKIP, NF-KBp65 and VEGF-C protein expression with clinical and pathological factors. The correlations among the three proteins were also analyzed. Results （ 1 ） RKIP expression positive rate in epithelial ovarian cancer tissues is 28. 13%, which is significantly lower than it in the normal ovarian tissues and benign ovarian epithelial tumor tissues （P 〈 0.01, P 〈 0.05 ）. NF-KBp65 protein expression positive rate in epithelial ovarian cancer tissue is 81. 25%, which is significantly higher than it in the normal ovarian tissue and ovarian benign epithelial tumor tissue （P 〈 0.01, P 〈 0.05 ）. VEGF-C expression positive rate in epithelial ovarian cancer tissue is 90.63%, which is significantly higher than it in the normal ovarian tissue and benign ovarian epithelial tumor tissue （ P 〈 O. 01, P 〈 O. 01 ）. （ 2 ） The expression of RKIP and NF-KBp65 in epithelial ovarian cancer has no correlation with the patients＇ age, histological type and pathological grade, but has correlation with the clinical stages （ P 〈 0. 05 ） ; The VEGF-C expression has no correlation with the patients＇ age, histological type, histological grade and clinical stages. （3）The expression of RKIP and NF-KBp65 in Epithelial ovarian cancer tissues is negatively correlated （r = -0. 4338, P = 0. 0131 ）. Conclusions RKIP, NF-KBp65 and VEGF-C may be involved in the occurrence and development of epithelial ovarian cancer. The RKIP play a role in the invasion and metastasis of epithelial ovarian cancer possibly through regulation of NF-KB signal pathway.
出处
《中国临床研究》
CAS
2013年第6期525-527,530,共4页
Chinese Journal of Clinical Research
