摘要
探讨反相微乳液法制备的β-纳米磷酸三钙/明胶/鹿茸多肽复合材料(简称纳米复合材料)对人成骨细胞功能的影响。利用反相微乳液法制备纳米复合材料,扫描电镜观察其形态,EDX与SELDI-TOF-MS分析对复合材料进行表征。采用MTT比色法、茜素红染色法检测纳米复合材料对体外培养的人成骨细胞hFOB1.19的增殖、矿化结节形成的影响;采用全自动生化分析仪检测hFOB细胞碱性磷酸酶活性(ALP)。结果表明该纳米复合材料可促进人成骨细胞增殖,提高ALP活性并增加矿化结节形成的数量,可促进人成骨细胞的增殖、分化成熟及矿化。
The nano tricalcium phosphate/gelatin/velvet antler polypeptide （VAP）composite ma- terials （referred as nanocomposites） were prepared by water-in-oil （W/O） emulsion process. The effect of nanocomposites on human osteoblasts was studied in hFOB1.19 cell lines. Nanocompos- ites were prepared through emulsification-coacervation method in water-in-oil emulsions. The mor- phology was observed by scanning electron microscope. Characterization of composite spheres was examined by EDX and SELDI-TOF-MS. MTT assay and alizarin red staining were employed to de- tect the effects of nanocomposites on the proliferation and formation of mineralization nodules for- mation in hFOB cells, respectively. The alkaline phosphatase（ALP） activity was determined by au- tomatic clinical chemistry analyzer. The results showed that nanocomposites could stimulate hu- man osteoblasts cells proliferation increase ALP activity,and promote the formation of mineraliza- tion nodules.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第6期898-904,共7页
Chinese Journal of Veterinary Science
基金
国家863计划资助项目(2006AA03Z328)
长春市科技局科技支撑计划项目(长科技合2008217号)
关键词
纳米磷酸三钙
明胶
鹿茸多肽复合材料
成骨细胞
增殖
分化
矿化
nano tricalcium phosphate/ gelatin /velvet antler polypeptide composites nanocomposites
human osteoblasts
proliferation
differentiation
mineralization
