摘要
为了研究杨树AP1同源基因的表达规律,利用PCR技术从毛果杨基因组DNA中克隆出PtrAP1-2基因上游一段2103bp序列。联合使用PLACE和PlantCARE在线软件分析序列,结果表明该序列含有TATA-box和CAAT-box启动子基本元件,另外含有MRE、GT1-motif、AE-box、TCT-motif和Box4多种光响应元件,因此,PtrAP1-2可能与植物开花过程紧密相关。在序列分析的基础上,构建了PtrAP1-2基因启动子驱动GUS报告基因的植物表达载体,命名为PtrAP1-2pro::GUS。利用农杆菌介导的瞬时表达法转化烟草,结果表明PtrAP1-2启动子可以驱动GUS基因在烟草萼片和花瓣中特异表达,在根、茎、叶、雄蕊和心皮等其他组织部位里没有GUS表达活性,这表明PtrAP1-2pro为花器官特异表达启动子。
In order to study the expression and regulation of AP1 homologous gene in poplar, a 2 103 bp 5＇ flanking sequence ofPtrAP1-2 gene was isolated by PCR from genomic DNA ofPopulus trichocarpa. Promot- er sequence analyzed by PLACE and PlantCARE showed that the sequence contains TATA-box, CAAT-box and many light-responsive elements, such as MRE, GTl-motif, AE-box, TCT-motif and Box 4. It was supposed that PtrAP1-2 was closely related to flower development. Based on sequence analysis, the PtrAP1-2 promoter was fused to the GUS reporter gene to characterize its expression pattem in tobacco. The results of Agrobacterium- mediated transient expression showed that the promoter could direct transgene expression in sepals and petals of tobacco, and no detection of GUS was found in roots, stems, leaves, stamens and carpels. It indicated that the PtrAP1-2 promoter was a floral organ specific promoter.
出处
《植物生理学报》
CAS
CSCD
北大核心
2013年第6期591-597,共7页
Plant Physiology Journal
基金
国家"863"计划(2013AA102703和2011AA100201)
国家自然科学基金(31170631)
"十二五"国家科技支撑项目(2012BAD01B0302)
关键词
毛果杨
AP1
启动子
GUS
瞬时表达
Populus trichocarpa Torr. ＆ Gray
AP1
promoter
GUS
transient expression
