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奶牛乳房炎多联菌苗抗体间接ELISA检测方法的建立

The establishment of indirect ELISA detection method on dairy cow mastitis multi-vaccine antibody
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摘要 奶牛乳房炎多联菌苗是一种安全、有效且免疫期长的新型疫苗,为了实时观测多联菌苗激发的金黄色葡萄球菌抗体水平及免疫持续期,建立了多联菌苗抗体间接ELISA检测方法。结果表明:在金黄色葡萄球菌抗原包被浓度为包被量5mg·L-1、血清稀释度为1∶200时,所建立的间接ELISA方法达到了最佳检测效果。 Dairy cow mastitis muLti - vaccine is a kind of new vaccine that has the advantages of safe, effective and immune period longer in order to observe real -time Staphylococcus aureus antibody level and immune period be multi -vaccine excitation and establish the indirect ELISA detection method of multi - vaccine antibody. The results showed that antigen coated concentration is 5mg . L-1 coating quantity, 1：200 serum dilution in Staphylococcus aureus, the indirect ELISA method achieved the best results.
作者 曲晓军 孙建华 李思明 高娃
机构地区 黑龙江省科学院微生物研究所
出处 《黑龙江科学》 2013年第5期84-85,41,共3页 Heilongjiang Science
关键词 奶牛乳房炎 多联菌苗 间接ELISA 检测 Dairy cow mastitis multi - vaccine indirect ELISA detection
分类号 S859.7 [农业科学—临床兽医学]
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参考文献7

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二级参考文献32

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黑龙江科学
2013年 第5期

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