摘要
采用反复硅胶柱色谱法和Sephadex LH-20凝胶色谱法分离纯化南海红树林内生真菌Gx-3a的代谢产物,并通过理化常数测定和光谱分析鉴定其化学结构。结果从南海红树林内生真菌Gx-3a的菌体中分离得到4个代谢产物:ditryptophenaline(1),3-hydroxy-4-(2,6,6-trimethyl-tetrahydro-2H-pyran-2-yl)benzoic acid(2),(E)-4,5-dihydroxy-3-(prop-1-enyl)cyclopent-2-enone(3),3,6-di-sec-butyl-1,4-dihy Droxypiperazine-2,5-dione(4),化合物1对口腔癌细胞KB,KBv200的抗肿瘤活性值分别为8.0和12.0μmol/ml。4个化合物均首次从南海红树林内生真菌Gx-3a中分离得到。
Secondary metabolites were studied from mangrove endophytic fungus No . Gx3a in the south china sea. The compounds were isolated by column chromatography, and identified on the basia of physic-chemical constants, and spectral analysis. Four metabolites were obtained and elucidated as ditryptophenaline（1）, 3-hydroxy-4-（2,6,6-trimethyl- tetrahydro- 2H-pyran-2- yl） benzoic acid（2）, （E）-4,5-dihydroxy-3-（prop-l-enyl） cyclopent-2-enone（3）, 3,6-di-sec-butyl- 1,4-dihydroxypiperazine-2,5-dione （4）. In the preliminary bioassay, ditryptophenaline showes strong inhibittory activity on the KB,KBv200 with LDso values of 8.0μMol and 12.0μMol, respectively. The four metabolites were obtained from endophytic fungus No · Gx-3a for the first time.
出处
《广西科学》
CAS
2013年第2期168-170,共3页
Guangxi Sciences
基金
国家自然科学基金项目(30973635)
中国科学院知识创新工程重要方向项目(KSCX2-YW-R-217)
广西教育厅科研项目(201204LX497)资助
关键词
代谢产物
内生真菌
红树林
metabolites, endophytic fungus, mangrove
