摘要
目的建立从枳实中分离制备柚皮苷和新橙皮苷对照品的方法。方法结合大孔树脂、中低压制备色谱分离制备枳实中柚皮苷和新橙皮苷单体。结果分离制备的柚皮苷的质量分数达98.76%,新橙皮苷的质量分数达99.50%。结论该方法可有效制备高纯度的柚皮苷和新橙皮苷,可用于制备中药定性、定量分析使用的对照品。
Objective To establish a separation method for naringin and neohesperidin reference substances from Citrus aurantium.Methods Naringin and neohesperidin monomers in C.aurantium were isolated and purified by macroporous resin and medium-lowpressure preparative chromatography.Results The contents of the prepared naringin and neohesperidin reached to 98.76% and 99.50%,respectively.Conclusion This method is effective for the preparation of naringin and neohesperidin with high purity.It could be used to prepare the reference substances for the qualitative and quantitative analyses of Chinese materia medica.
出处
《中草药》
CAS
CSCD
北大核心
2013年第10期1271-1274,共4页
Chinese Traditional and Herbal Drugs
基金
国家科技支撑计划项目(2007BAI37B07)
关键词
柚皮苷
新橙皮苷
枳实
对照品
中低压制备色谱
naringin
neohesperidin
Citrus aurantium L.
reference substance
medium-low-pressure preparative chromatography
