摘要
以质粒 pc DNA3和 p SV2 -dhfr为基础 ,构建了 2个通用表达载体 p MCD-A和 p MCD-B,然后将 EPOminigene克隆于它们的多克隆位点之中 ,得到表达载体 p AE和 p BE,经脂质体导入 CHO-dhfr- 细胞瞬时表达 ,结果表明 ,p MCD-A和 p MCD-B表达效果较好 ,表达产物经网织红细胞法测定 。
To achieve high expression of heterologous gene, two universal eukaryotic vectors: pMCD A and pMCD B were constructed from pcDNA3 and pSV2 dhfr. Then EPO minigene were cloned into their multiple cloning sites. The recombinant plasmids were pAE and pBE. They were transfected into CHO dhfr - cells by lipofectin respectively. Their transient expression showed that they can express heterologous gene in CHO dhfr - cells efficiently. By reticulocyte counting, all the secreted EPO revealed their biological activity in vivo.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2000年第3期243-245,共3页
Chinese Journal of Veterinary Science
基金
国家"8 63"计划资助项目! ( Z2 1-0 4-0 3 )
