摘要
以固定化脂肪酶Lipozyme RM IM为催化剂,研究无溶剂体系下樟树籽油和甘油合成中链脂肪酸甘油二酯的工艺。采用响应面分析法对甘油酶解反应的工艺条件进行优化,确定最佳条件为底物摩尔比(甘油∶樟树籽油)=1∶2.3,加酶量11.7wt%,反应温度72℃,反应时间15h。在此条件下得到产物油中甘油二酯含量为51.60%。气相色谱法测定合成产物的脂肪酸组成,产物油中癸酸(C10∶0)及月桂酸(C12∶0)含量分别为54.68%和39.79%,与樟树籽油脂肪酸组成基本一致。合成产物中DAG的中链脂肪酸含量虽有一定降低,但总量仍能达到75%左右。
The lipase-catalyzed reaction of Cinnamomum camphora seed oil （CCSO） and glycerol was studied to produce diacylglycerol（DAG） rich in medium-chain fatty acid（MCFA） in a solvent free system using Lipozyme RM IM immobilized lipase as the catalyst. Response surface methodology was used to optimize the reaction conditions of glycerolysis. The optimal parameters were as follows .. substrate molar ratio of glycerol to CCSO was 1：2.3,enzyme load was 11.7wt%, reaction temperature was 72℃, and reaction time was 15h. Under the optimal conditions,DAG content could reach 51.60% in the reaction product. Fatty acid compositions of the reaction products were detected by the gas chromatographic. The contents of capric acid （C10：0） and lauric acid（C12：0） in the DAG oil were 54.68% and 39.79%,which remained the similar fatty acid composition as CCSO. Though the total content of MCFA in DAG of the reaction product deceased in some extent,it could still reach about 75%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第12期111-114,共4页
Science and Technology of Food Industry
基金
中国博士后面上资助第51批(2012M511457)
江西省自然基金(20132BAB204001
20122BAB214001)
国家自然基金(31071561)
关键词
甘油二酯
樟树籽油
酶法甘油解
中链脂肪酸
diacylglycerol
Cinnamomum camphora seed oil
enzymatic glycerolysis
medium-chain fatty acid
