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粗柠檬与哈姆林甜橙二倍体体细胞杂种胞质基因组初步分析 被引量:6

Preliminary Analysis of Cytoplasmic Genome of Diploid Somatic Hybrid Derived from Fusion Between Rough Lemon and Hamlin Sweet Orange
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摘要 RFLP (restriction fragment length polymorphism) was employed to analyze cytoplasmic genome of diploid somatic hybrid plant, morphologically similar to rough lemon which was leaf parent, that was produced via protoplast fusion between rough lemon ( Citrus jambhiri Lush) and Hamlin sweet orange ( C. sinensis Osb.), the embryogenic parent. Three enzyme_mitocondrial probe combinations and one enzyme_chloroplast probe combination demonstrated that the plant had identical band profiles to Hamlin sweet orange as far as mtDNA and cpDNA were concerned. RFLP (restriction fragment length polymorphism) was employed to analyze cytoplasmic genome of diploid somatic hybrid plant, morphologically similar to rough lemon which was leaf parent, that was produced via protoplast fusion between rough lemon ( Citrus jambhiri Lush) and Hamlin sweet orange ( C. sinensis Osb.), the embryogenic parent. Three enzyme_mitocondrial probe combinations and one enzyme_chloroplast probe combination demonstrated that the plant had identical band profiles to Hamlin sweet orange as far as mtDNA and cpDNA were concerned.
出处 《Acta Botanica Sinica》 CSCD 2000年第1期102-104,共3页 Acta Botanica Sinica(植物学报:英文版)
基金 国家自然科学基金!重点项目 ( 3 9870 5 19 3 962 5 0 17)&&
关键词 柑橘 胞质杂种 RFLP 线粒体基因组 原生质体融合 citrus hybrid RFLP mitocondrial genome
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