摘要
Compatible and incompatible reactions in rice plants ( Oryza sativa L. cv. Shenxianggen No.4) were resulted from inoculation with two different virulent races of rice blast fungus ( Magnaporthe grisea (Hebert) Barr), and thus an effective infecting system was established between rice plants and the rice blast pathogen. Two cDNA clones that showed induced and temporal patterns in expression in the very early stage in response to infection of the fungus were obtained from the plants by use of differential display. Of the two cDNA clones, Fastresp_a was induced to express in both compatible and incompatible interactions although it was expressed earlier in the former reaction. The second one, Fastresp_b , was only expressed in incompatible interaction. Southern blot analysis of the rice genomic DNA indicated that both of the two clones were from genome of the plant. No significant homology to the two genes was found from the rice gene database. This suggested that they were novel genes in rice and may play important roles in rice resistant response to infection of rice blast fungus.
以亲和性与非亲和性两个稻瘟病原真菌小种 (Magnaporthegrisea (Hebert)Barr)感染同一水稻品种 (OryzasativaL .cv .ShenxianggengNo .4)的植株产生明显不同的致病和抗病反应 ,由此建立了有效的感染系统。应用区分显示技术获得两个在侵染早期具有诱导表达特征的cDNA克隆 ,其中一个同时在致病和抗病反应中进行早期诱导表达 ,但在抗病反应中的诱导相对早于其在致病反应中的诱导表达。而另一克隆则只在抗病反应中进行早期诱导表达。基因组DNA杂交表明这两个cDNA克隆均来自水稻的基因组。顺序分析表明在水稻的基因数据库中均没有它们的同源序列 ,它们在水稻对稻瘟病原菌侵染的抗性反应中可能起重要作用。
基金
ThisworkwasfinanciallysupportedbyNationalLaboratoryofPlantMolecularGenetics,ShanghaiInstituteofPlantPhysiology ,TheChineseAc
