摘要
为查明维生素C二步发酵混合培养中巨大芽孢杆菌与氧化葡萄糖酸杆菌间的关系,通过生长曲线测定、静息细胞实验及摇瓶发酵实验研究了巨大芽孢杆菌对氧化葡萄糖酸杆菌生长和产生2酮基L古龙酸作用的影响;采用超滤分离、凝胶层析及聚丙烯酰胺凝胶电泳技术对巨大芽孢杆菌胞外液中具有促进氧化葡萄糖酸杆菌产酸作用的活性物质进行了分离和纯化.结果表明,大菌胞内液和胞外液均可促进小菌生长,大菌胞外液中具有该作用的组分分子量在100KDa以上;大菌胞外液具有促进小菌转化L山梨糖生成2酮基L古龙酸的作用,其胞内液无该作用;大菌胞外液中具有该活性作用的组分为30～50KDa和大于100KDa两个部分.其中30～50KDa范围的大菌胞外液中的活性组分为一种蛋白质,其表观分子量约为35KDa,由一种亚基构成。
To reveal the relationship between Bacillus megaterium and Gluconobacter oxydans in the mixed culture of vitamin C two step fermentation,the effect of B.megaterium on the growth of G.oxydans and its synthesizing ability of 2 keto L gulonic acid(2KGA) was studied.The bioactive metabolites which could enhance the synthesis of 2KGA were isolated and purified by ultrafiltration,gel chromatography and SDS polyacrylamide gel electrophoresis.Both the culture supernatant and the cytosol of B.megaterium could promote the proliferation of G.oxydans ,and the active component in the culture supernatant was above 100 KDa.The culture suernatant could enhance the conversion of L sorbose to 2KGA,while the cytosol could not.The active components in B.megaterium culture supernatant had molecular weight of 30～50 KDa and above 100 KDa,and the former was a kind of protein with an apparent molecular weight of about 35 KDa,which consisted of one sort of subunit and contained Fe and Zn elements.
出处
《应用生态学报》
CAS
CSCD
2000年第1期119-122,共4页
Chinese Journal of Applied Ecology
基金
中国科学院"百人计划"资助项目
关键词
氧化葡萄糖酸杆菌
巨大芽孢杆菌
混合发酵
VC
Mixed culture, Gluconobacter oxydans, Bacillus megaterium, Proliferation, 2 keto L gulonic acid.
