摘要
采用 PCR技术 ,从我国广泛栽培甘薯品种南薯 88基因组中扩增和克隆到甘薯贮藏蛋白 A基因编码区段 ,并测定了其全部核苷酸序列 .该编码区长 65 7bp,编码一个长 2 1 9个氨基酸残基的蛋白质 ,其中信号肽长 37个氨基酸残基 ,成熟蛋白质长 1 82个氨基酸残基 ,其分子量为 2 0 k D.将该片段的核苷酸序列与已登录在 Gen Bank中的另外 6个甘薯贮藏蛋白 A基因编码区序列进行比较 ,发现其同源性高达 90 % ,说明甘薯贮藏蛋白 A基因编码区序列具有高度保守性 .虽然 7个基因编码区的核苷酸总变异为 1 0 % ,但在每两个基因之间的比较则表明其核苷酸的变异范围小于 7% .
The coding region of sporamin A gene from sweet potato Nanshu 88,a widely cultivated variety in China,was amplified by the polymerase chain reaction (PCR) with primers designed on the sequence of sporamin gene gSPO Al.The amplified fragment was cloned and sequenced.This coding region was 657 bp in size and encoded a peptide of 219 amino acid residues,in which there was a signal peptide with 37 amino acids and a mature peptide of 182 amino acids.The molecular weight of the mature sporamin was 20 kD.Comparison of this sequence with those of other six sporamin A genes in GenBank showed that the nucleotide homology was as high as 90%,indicating that the sequence of sporamin coding region was very conserved.Although the total variety among all seven sporamin genes was about 10% the variety between each two sporamin genes was less than 7%.
出处
《中国生物化学与分子生物学报》
CSCD
2000年第1期41-45,共5页
Chinese Journal of Biochemistry and Molecular Biology
基金
四川省"九五"重点科学技术研究资助项目
