摘要
以含有虹鳟鱼金属硫蛋白基因的质粒DNA为模板 ,以合成的两段 32个寡聚核苷酸为引物 ,经过PCR扩增 ,合成了 4 33bp的片段 ,把其克隆到pBL -CAT载体中 ,序列分析和限制酶切图谱表明所克隆的虹鳟鱼金属硫蛋白启动子含有 4 33bp ,含有TATA和CAAT序列 ,与Hong报导的鳟鱼金属硫蛋白启动子的序列比较 ,其核苷酸的同源率为 10 0 %。
Plasmids ptMTA contain metallothionein gene of rainbow trout as template, synthetic 32 oligonucleotides as primer, a fragment which contain 433 base pairs was amplified by PCR and was cloned into vector pBL CAT, The result of sequence analysis and restriction map shows metaothionein pomoter of rainbow trout contains 433 base pairs, TATA and CAAT sequence. Compared with Hong's metallothionein promoter of rainbow trout, homology of nucleotide of metallothinein promoter for rainbow trout is 100%
出处
《生物技术》
CAS
CSCD
2000年第1期1-3,共3页
Biotechnology
