摘要
In the cultivation of gene engineered strain of Escherichia coli on glucose medium, excretion and accumulation of acetic acid inhibit not only cell growth but also the the expression of heterologous protein. It is obvious that the desirable host strain maintaining acetate at a low level is one of the approaches to increase the production of recombinant protein. The present article deals with the selection of mutants of E.coli DP19, DP8, which grow on the medium containing pyruvate as the sole carbon source in the presence of 50mmol/L fluoroacetic acid. It is shown that mutant DP19 is defective in its phosphotransacetylase(PTA)activity and accumulates less acetate in the medium, while DP8 is defective in acetate kinase (ACK)and accumulates similar level of acetate comparing with its parent. Using pta - mutant E.coli DP19 as host, the expression of GL 7ACA acylase gene on the recombinant plasmid pMR24 is improved, and the yield of enzyme activity in flask fermentation is about twice as much as its parent.
In the cultivation of gene engineered strain of Escherichia coli on glucose medium, excretion and accumulation of acetic acid inhibit not only cell growth but also the the expression of heterologous protein. It is obvious that the desirable host strain maintaining acetate at a low level is one of the approaches to increase the production of recombinant protein. The present article deals with the selection of mutants of E.coli DP19, DP8, which grow on the medium containing pyruvate as the sole carbon source in the presence of 50mmol/L fluoroacetic acid. It is shown that mutant DP19 is defective in its phosphotransacetylase(PTA)activity and accumulates less acetate in the medium, while DP8 is defective in acetate kinase (ACK)and accumulates similar level of acetate comparing with its parent. Using pta - mutant E.coli DP19 as host, the expression of GL 7ACA acylase gene on the recombinant plasmid pMR24 is improved, and the yield of enzyme activity in flask fermentation is about twice as much as its parent.
出处
《微生物学报》
CAS
CSCD
北大核心
2000年第1期100-104,共5页
Acta Microbiologica Sinica
基金
国家"九五"攻关项目
上海市现代生物与新药产业发展基金
关键词
氟乙酸
GL-7ACA酰化酶
抗性菌株
选育
Fluoroacetic acid, Acetic acid, phosphotransacetylas (PTA), Acetate kinase (ACK),GL 7ACA acylase
