摘要
将西瓜野生种质ＰＩ２９６３４１抗枯萎病生理小种１的抗性基因连锁的ＲＡＰＤ标记ＯＰＰ０１／７００进行克隆、测序，Ｓｏｕｔｈｅｒｎ杂交证明此标记为１个单拷贝，并转化为ＳＣＡＲ标记，简化了ＳＣＡＲ扩增产物的检测技术。上述技术在抗病转育后代选择中得到了很好的应用，初步建立了西瓜抗枯萎病育种分子标记辅助选择技术系统。
The RAPD marker OPP01 / 700 proved to be linked to the Fusarium wilt resistant gene in watermelon wild germplasm PI296341 was cloned and sequenced, and transferred into a SCAR marker. The linked marker was tested to be one copy by Southern blotting. The technique of detection of the SCAR--amplified products was simplified. These techniques were applied in selecting resistant plants in F3 of the introgression of the disease resistant gene. It is the first report in this field of transfer ring a RAPD marker linked to disease resistance gene into SCAR marker, and establishing the technique of molecular marker-- assisted--selection for breeding Fusarium wilt resistance cultivars.
基金
国家自然科学基金!39670514
北京科技新星计划资助!951872300
关键词
西瓜
枯萎病
RAPD
SCAR
抗病育种
watermelon (Citrullus lanatus)
Fusarium wilt resistance, SOAK
RAPD
molecular marker-assisted--selection
