摘要
以２５６个随机引物对含小麦抗白粉病基因Ｐｍ２近等基因系进行ＲＡＰＤ分析，发现１７个随机引物的扩增产物在抗、感ＮＩＬｓ材料间表现多态性，且其中５个引物经４次以上重复，均获相同结果，其多态性标记分别为ＯＰＭ０８(１６００)、ＯＰＩ０４(１７００)、ＯＰＨ１９(１１００、ＯＰＥ０９(９００)及ＯＰＭ１６(８５０)。当以这５个随机引物对１４个已知含Ｐｍ２基因的抗病材料及９个不含Ｐｍ２基因的感病材料进行检测时，只有标记ＯＰＩ０４(１７００)在１２个抗病材料中出现（另两个抗病材料中未检测到），而在９个感病材料中均未出现。进一步用 ＯＰI(０４)对１０２株（Ｃｈａｎｃｅｌｌｏｒｘ Ｕｋａ／８＊Ｃｃ）Ｆ２分离群体进行分析，估算出标记ＯＰＩ０４(１７００)与Ｐｍ２基因间的遗传距离为１２．２±３．３ｃＭ。
The random amplified polymorphic DNA (RAPD) analysis was made in Pm2 near-isogenic lines (NILs) with 265 random primers. Seventeen out of the 256 tested primers amplified the polymorphic DNA in the NILs. Five of them showed the same discriminating results in more than four replications. The polymorphic bands were assigned to be OPM081600, OPI041700, OPH19lloo and OPE09900, respectively. Using these five polymorphic Primers to detect 14 resistant materials with Pm2 and 9 susceptible matarials without Pm2, only OPI041700 was amplified in 12 of the 14 resistant materials and absent in all of the 9 susceptible materials. Further RAPD analysis of (Chancellor x Ulka/8*Cc)F2 plants by primer OPI04 indicated that the genetic distance of OPI041700 to Pm2 was 12.2 3.3cM.
基金
国家自然科学基金!39770413
国家‘863’计划(Z-17-04-01)资助
关键词
小麦
近等基因系
RAPD标记
白粉病
抗病育种
wheat, near isogenic line
powdery mildew resistance gene (Pm)
RAPD marker
