摘要
应用特异于ＨＭＧ－ｂｏｘ区域的兼并引物，扩增了大鳞副泥鳅的Ｓｏｘ基因。在扩增产物中 发现３条大小分别为２２０ｂｐ． ５５０ｂｐ和１５００ｂｐ的扩增带。经克隆和ＤＮＡ序列分析，从片段 ５５０ｂｐ扩增带中得到一个新的基因片段，其可能编码的蛋白质氨基酸序列与人、鸡、小鼠的 Ｓｏｘ９基因相似性最高，达到９４％；其与ＨＳＯＸ１０、ＭＳｏｘ１０、ＨＳＲＹ、ＭＳｒｙ的相似性分别为８７％、 ５５％、４０％和４７％。根据这些特性把其命名为ＰｄＳｏｘ９ Ｎｏｒｔｈｅｒｎ杂交结果表明ＰｄＳｏｘ９在成体 脑及精巢组织中有宏量表达。
Degenerate primers within HMG--box analyzed the box genes of P. dabryanus. Three amplification bands with the length 220, 550 and 1500hp respectively were observed. A novel gene was obtained from the 550hp band. The identity of possible amino acid sequence to Sox9 of human, mouse and chicken is 94%. The similarity to HSOX10, Msox10, HSRY MSry is 87%, 55%, 40% and 47% respectively. So, it was named PdSOx9. Northern blotting result showed that it overexposes in adult testis and brain.
基金
国家自然科学基金!39770407
