摘要
采用聚丙烯酰胺梯度凝胶电泳及特异性组织化学染色技术分析了鲤、鲫及其基因组叠加的不同组合个体的红细胞ＳＯＤ． ＥＳＴ和血红蛋白的电泳图谱。结果表明不同基因组组合及不同倍性的个体间存在由基因组差异导致的生化多态性，为不同倍性的鱼类的同工酶（ＳＯＤ）表达存在基因剂量效应提供了依据，证实了不同组合的ＳＯＤ亚基间有协同表达、累积作用。研究表明每个基因有其独特的调控机制，因而在杂种三倍体内三分之一的基因组亦能够表达产物。应用本研究的遗传学数据有益于构建基因组特异性品系，这不但有助于更有效地鉴别鱼类的不同组合而且为深入研究基因剂量和表达调控的关系奠定了基础。
The patterns of SOD, EST and haemoglobin genes expression among the different combinations conducted with genome addition were investigated. The erythrocyte expression of these isozymes and protein were visualized by histochemical staining after polyacrylamide gradient gel electrophoresis. The results of our electrophoresis analysis have revealed that biochemical polymorphism exists in the combinations, and these genetic variance have resulted from the contribution of different genomes. This study provides additional evidence that gene dosages can be interpreted from the enzyme patterns and electrophoretic staining intensities existed in different ploidies, which suggests that subunit interactions for SOD are co--operative and additive. This would indicate that each allele could be differentially regulated so that even one--third genome can be expressed in triploid individuals. These genetic data could be used to construct genome specific varieties, and would not only help to maximize efficiency in the use of different biotypes identification, but also help to maximize studying the relationship between gene dosage, expression and regulation.
基金
国家自然科学基金!39823003
