摘要
Ｎｏｄａｌ基因属于 ＴＧＦ－β超家族。它在小鼠原肠期的原条形成过程中起重要作用。 Ｎｏｄａｌ 基因的缺失导致中胚层不能形成。使小鼠死亡。用一系列含有 Ｎｏｄａｌ基因 ５’侧翼序列其缺失 体的荧光素酶报告基因质粒瞬时转化Ｆ９细胞。通过分析这些报告基因质粒的荧光素酶活性， 在转录起始点上游的１ｋｂ处的ＥｃｏＲＩ附近找到一个抑制子元件。该抑制子元件的核心部分 位于ＥｃｏＲＩ位点下游约２００ｂｐ处，它几乎可以完全抑制Ｎｏｄａｌ基因启动子的活性。但对于 ＳＶ４０启动子没有影响。在不同的细胞种类中它的作用不同，在ＣＨＯ细胞中它具有增强活性。
Nodal gene is a member of the TGF superfamily. It is required for the formation of the primitive streak during mouse gastrulation. Mice without Nodal gene will die due to the lack of mesoderm formation. F9 cells were transiently transfected with a series of luciferase reporter constructs containing the subcones of Nodal 5' filanking sequences and their deletions. By analyzing the luciferase activity of these constructs, a suppressor element was determined at about 1kb up stream the transcription initiation site, around an EcoRI site. Its centrol part was located at sbout 100hp downstream of that EcoRI site. It could almost totally, suppress the Nodal gene promoter activity, though it had no effect on SV40 promoter. It behaved differently in different cell lines. In CHO cells, it had an activation activity.
基金
国家自然科学基金!39470371
