摘要
利用抗生素干扰小鼠肠道原籍菌群,建立小鼠肠道菌群失衡模型;并利用乳酸菌和双歧杆菌制剂对肠道菌群失调小鼠模型进行调节治疗。观察乳酸菌和双歧杆菌对肠道菌群失调模型的调节效果。选用不同浓度的头孢曲松钠,对小鼠进行灌胃。取盲肠和结肠内容物培养主要菌群变化。模型制备成功后利用乳酸菌和双歧杆菌进行菌群的治疗性调节,口服6周后,检测被检菌群的数量。结果不同剂量头孢曲松钠灌胃的小鼠盲肠体积均有所增大,模型组的优势菌的数量显著减少。通过口服乳酸菌和双歧杆菌制剂,可以适当的恢复乳酸菌和双歧杆菌的数量。与对照组比较差异显著。说明实验成功地制备了小鼠的肠道菌群失衡模型,模拟长期应用抗生素的机能环境,通过外源性的补充乳酸菌和双歧杆菌,对保持肠道菌群的平衡具有调节和修复作用。
Use of antibiotics interference mice intestinal bona fide flora, the establishment of your degree of flo- ra imbalance mice model. Use of lactobacillus and bifidobacterium to intestinal dysbaeteriosis mice regulation. Ob- servation lactic acid bacteria and bifidobacteria on intestinal flora disequilibrium model regulation effect. Different concentration of ceflriaxone, the mice lavage is Choosed. Cecum content culture main flora changes is take. After the success of the model for the preparation of using lactic acid bacteria and bifidobacteria in flora of therapeutic ad- justment, oral 6 weeks after the test, the flora number is detected. Different dosages ceftriaxone lavage in mice have been cecum volume increases. The advantage of the model group was significantly reduced in the number of bacteri- a. Through oral lactobaeillus and bifidobaeterium preparation, can proper recovery lactobacillus and bifidobacterium quantity. Significant difference compared with controls. It is conclused that experiment successful preparation mouse intestinal flora imbalance simulation model, long-term use of antibiotics, the function of the environment through the external supplement lactic acid bacteria and bifidobacteria, to keep the balance of the intestinal flora has regula- tion and repair action.
出处
《科学技术与工程》
北大核心
2013年第16期4497-4500,共4页
Science Technology and Engineering
关键词
乳酸菌
双歧杆菌
抗生素
菌群失调
bifidobacterium
Lactobacillus
flora disequilibrium
animal model
