摘要
目的探讨血管内皮生长因子(VEGF)R-3和CD34标记的阳性脉管在宫颈癌组织中的表达特点,分析其与宫颈癌临床病理特点之间的关系。方法选65例宫颈鳞状细胞癌患者的癌组织和癌灶边缘组织,采用双标和单标免疫组化方法,检测65例宫颈鳞癌组织VEGFR-3和CD34标记阳性脉管的密度。另选10例其他因良性疾病切除子宫得到的宫颈组织为对照组。结果癌灶边缘组织中VEGFR-3阳性脉管密度显著高于癌组织(P<0.05),也显著高于对照组宫颈组织(P<0.05);癌灶边缘组织和癌组织中CD34阳性脉管密度差异无统计意义(P>0.05),均高于对照组宫颈组织(P<0.05)。Ⅱa期组癌灶边缘组织中VEGFR-3和CD34阳性脉管密度分别为(17.35±2.67)和(16.97±3.13),显著高于Ⅰb期组的(12.32±1.24)和(12.46±3.27)差异无统计学意义(P<0.05);淋巴结转移组癌灶边缘组织VEGFR-3阳性脉管密度为(18.68±0.89),显著高于无淋巴结转移组的(12.36±3.48)差异无统计学意义(P<0.05),CD34阳性脉管密度在两组中表达差异无统计学意义(P>0.05)。结论宫颈癌组织中VEGFR-3和CD34的表达与宫颈癌临床分期有关,癌灶边缘组织的VEGFR-3高表达与淋巴结转移密切相关。
Objective To research the characteristics of expressions of vascular endothelial growth factor receptor-3（VEGFR-3） and CD34 labeled positive vessels in tissue of cervical squamous cell cancer, analyze the relationship between their expression and clinicopathological characteristics of cervical squamous epithelial cell cancer.Methods Double-labeling and single-labeling immunohistochemistry were used to detect the densities of VEGFR-3 and CD34 labeled positive vessels in 65 cases with cervical cancer. Results The densities of VEGFR-3 labeled positive vessels in adjacent tissue of cervical cancer were significantly higher than those in cervical cancer tissue（P 0.05） and cervical tissue in control group（P 0.05）. there was no statistically significant difference in the density of CD34 labeled positive vessels in adjacent tissue of cervical cancer and cervical cancer tissue（P 0.05）;but both were significantly higher than that in cervical tissue in control group（P 0.05）.The densities of VEGFR-3 and CD34 labeled positive vessels in adjacent tissue of cervical cancer of stage Ⅱa were（17.35 ± 2.67） and（16.97 ± 3.13）,which were significantly higher than those in adjacent tissue of cervical cancer of stageⅠb（12.32 ± 1.24） and（12.46 ± 3.27）.The densities of VEGFR-3 in adjacent tissue of cervical cancer in lymph node metastasis group were（18.68 ± 0.89） significantly higher than those in adjacent tissue of cervical cancer in non-lymph node metastasis group（12.36 ±3.48）,there was no statistically significant difference in the density of CD34 labeled positive vessels between the two groups（P 0.05）.Conclusions The expressions of VEGFR-3 and CD34 in cervical cancer are correlated with clinical stage of cervical cancer,the high expression of VEGFR-3 in adjacent tissue of cervical cancer are related to lymph node metastasis closely.
出处
《中国肿瘤临床与康复》
2013年第5期446-448,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
