摘要
目的 :研究肾上腺髓质素 (AM)在缺氧性肺动脉高压 (HPHT)演进过程中含量的变化规律及其与ET 1的关系。方法 :应用常压间断缺氧法建立大鼠HPHT模型 ,用放免法测定对照组和不同缺氧时间组血浆和肺组织AM浓度以及血浆ET 1浓度 ,并用免疫组化方法观察不同组大鼠肺AM的分布和含量变化。结果 :缺氧 3d起血浆AM浓度增高 ,1 4d起进一步增高 ;ET 1 /AM值自缺氧第 1天起增高 ,7～ 1 0d达到高峰 ;肺组织AM浓度缺氧 1 4起升高 ;免疫组化示AM样物质主要沉积于血管 ,缺氧 1 4～ 2 1d组染色颗粒明显增多。结论 :AM可能在HPHT演进过程中起重要作用。
OBJECTIVE:To study the variation of adrenomedullin(AM) concentration and its relation to ET 1 in the development of hypoxic pulmonary artery hypertension(HPHT).METHOD:HPHT rat models were established by intermittent nomobaric hypoxia and The concentrations of AM in serium and lung tissue as well as The concentration of ET 1 in serium were measured by radioimmunoassay.Distribution and changes of AM in lung tissue were observed immunohistochemically.RESULTS:AM serium concentration increased from the 3 rd day of hypoxia and became higher from the 14 th day on while value of ET 1/AM increased on 1 st day of hypoxia and reached its peak on the 7～10 th day.AM like immunoreactive fibres were mainly localized within pulmonary vascular vessels and increased significantly in the 14～21 st day of hypoxia.CONCLUSION:AM may be an important factor relating to pathogenesis of HPHT.
出处
《中国现代应用药学》
CAS
CSCD
2000年第3期180-183,共4页
Chinese Journal of Modern Applied Pharmacy
关键词
肾上腺髓质素
缺氧性肺动脉高压
内皮素
adrenomedullin,hypoxia,pulmonary arteries,hypertension,endothelin
