摘要
目的研究微波辐射对大鼠回肠上皮细胞形态结构和肥大细胞(mast cell,MC)的影响。方法健康雄性SD大鼠20只,随机均分为对照组(常规饲养)和辐射组(常规饲养+辐射)。微波信号发生器规格:900MHz,1.0W,辐射频率为214μm/cm3。观察辐射过程中大鼠体质量变化,光镜观察回肠组织黏膜小肠绒毛的形态学改变,透射电镜观察回肠组织细胞超微结构的变化,甲苯胺蓝染色观察MC的数目变化。结果辐射组的大鼠体质量增加减缓;小肠绒毛结构紊乱,绒毛结构基本消失,小肠腺排列紊乱,肠腺间距增宽,肌层变薄,小肠黏膜细胞边界不清,核仁消失,线粒体等细胞器空泡化,边界不清,胞质内含大量脂滴,杯状细胞内空泡化。辐射组MC显著多于对照组,差别有统计学意义(P<0.05)。结论微波辐射可导致大鼠回肠上皮细胞形态结构的病理学改变,引起回肠MC数目明显增多。
Objective To study the morphological changes of the epithelial cells from rats＇ ileum treated by microwave radiation and the effect of microwave radiation on the mast cell （MC）. Methods SD-rats（ ♂ , 20） were randomly divided into two groups： experiment group and control group. The rats of experiment group were given microwave radiation （900 MHz, 1.0 W, 214μm/cm^3） while those of con- trol group were conventionally bred. We observed the body weight and the morphological changes of the ileum mucosa, after which the ultrastructure of the specimens was observed under transmission electron microscopy, and mast cells distribution was detected with toluidine blue stain. Results Deduction in body weight was observed from the rats of experiment group when compared with the control group. In experiment group, the structure of the intestinal villi was disorderd; the villous structure disappeared; the intestinal gland was disorganized with the spacing widened in midgut gland and myometrial thinning. Intestinal mucosa cells were poorly circumscribed with the disappearance of nucleolus and the structure of cells like mitochondria and goblet cells were vacuolate. Fat was accumulated in the cytoplasm of small in testinal mucosal epithelial cells. The number of mast cells in the experimen group was larger than that in the control group （P〈0.05）, lead to pathological changes in ileum. which was of clinical significance. the morphology of epithelial cells and Oonclusion Microwave radiation may significant increase of mast cells in rat
出处
《福建医科大学学报》
2013年第1期21-24,共4页
Journal of Fujian Medical University
基金
福建省科技计划重点项目(2012Y0032)
关键词
微波辐射
回肠
肥大细胞
microwave radiation
ileum
mast cell
