摘要
采用高效液相色谱 (HPL C,带有二级管阵列检测器 )测定化妆品的杀菌剂三氯生。用 C1 8柱 ,以乙腈 -水 (6 0∶ 40 ) ,用冰醋酸调节 p H值 3.0为流动相 ,检测波长 2 80 nm,以保留时间及紫外光谱图作定性 ,以峰面积或峰高作定量。该方法准确度和精密度较高 ,各类化妆品加标平均回收率范围为 91.9%～ 98.8% ,相对标准偏差范围为 0 .72 %～ 2 .8%。
A method was developed for the determination of an antiseptic\|trichlosan in cosmeticy by HPLC followed by diode\|array detection.The chromatographic separation was performed on C 18 column with a mobile phase of acetonitrile\|water(60∶40,V/V)which was adjusted to pH3 0 by glacialacetic acid.The UV wavelength was 280nm.Retention time as well as UV spec\|trum were used for the qualitation of trichlosan,and peak area or peak height for quantitation.The method showed good accuracy and precision average recovery of trichlosan from different cosmetics was between 91 86% and 98 83%.Relative standard deviation was between 0 72% and 2 75%.
出处
《卫生研究》
CAS
CSCD
北大核心
2000年第3期154-155,共2页
Journal of Hygiene Research
关键词
高效液相色谱
杀菌剂
三氯生
化妆品
测定
high\|performance liquid chromatography, diode\|array detection,cosmetics, anti\|bacterial agents, trichlosan
