摘要
为了探索鼠尾肌腱胶原蛋白在实验医学中的应用，用简单的方法溶解新鲜大白鼠鼠尾肌腱，从中提取较纯的胶原蛋白，制成胶原凝胶。将提取的胶原蛋白液进行定性、定量鉴定。结果：提取液主要为Ⅰ型胶原蛋白；72小时37℃恒温培养箱内培养无细菌生长；紫外分光光度计测定，吸收高峰为230nm；氨基酸成分主要为甘氨酸：占40％．辅氨酸：14．3％。显微镜下见：相互交织的胶原原纤维结构组成的网状结构；纤维均匀．有规则横纹。胶原蛋白液在37℃恒温下，可制成凝胶，用于培养细胞；用磷酸液冲洗胶原蛋白凝胶可成固体片状，制成胶原膜，用作医用材料。
in order to explore application of collagen from rat rail tendon to experimental medicines, the collagen, extracted from solvating fresh rat rail tendons by using a simple economical method, has been prepared collagen gel. The collagen solution identify with quantitative and qualitative analyses. Result show: the solution, extracted from rat rail tendons, consist mainly of type I collagen;No growth of bacteria in 37t therrnostatic culture box for 72 hours;Absorbing the height of ultraviolet-spectrophotometry is 230 nm by using flicker photometer examination; Amino acid in type I collngen consist of aminoacetic acid(40 per cent) and proline(14. 3 per cent); Under microscope: collngen gel netted constraction consist of original collagen fibril that had been fairly well-distributed and had a quite regular striate lines; The collagen solution prepared collagen gel matrixes in 37℃ thermostasis for culture cells and also prepared collagen solid film by washing collagen gel with a phosphate solution for plasty surgery materials.
出处
《海南医学》
CAS
2000年第3期64-65,共2页
Hainan Medical Journal
