摘要
目的研究白藜芦醇对小鼠CD4+T细胞活化的影响。方法将BALB/c鼠(SPF,雌性,9～10周龄)脾脏单个核细胞与不同浓度(0、10、20、40 mmol/L)的白藜芦醇共同孵育30 min后,分别采用刀豆蛋白A(ConA)、抗CD3抗体、抗CD3/抗CD28抗体刺激继续培养;分别共培养6 h、36 h后收集细胞采用单克隆抗体抗CD4-PerCP和抗CD69-FITC或抗CD71-FITC染色后,流式细胞术检测CD4+T细胞CD69、CD71的表达。结果白藜芦醇(≥10 mmol/L)可显著抑制抗CD3抗体引起的CD4+T淋巴细胞CD69表达(P<0.01),其抑制作用呈剂量依赖关系。白藜芦醇(≥20 mmol/L)可显著抑制由ConA、抗CD3/CD28抗体引起的CD4+T淋巴细胞CD69表达(P<0.05),其抑制作用呈剂量依赖关系。白藜芦醇(≥10 mmol/L)可显著抑制ConA引起的CD4+T淋巴细胞CD71表达(P<0.05),其抑制作用呈剂量依赖关系。白藜芦醇(≥20 mmol/L)可显著抑制由抗CD3抗体、抗CD3/抗CD28抗体引起的CD4+T淋巴细胞CD71表达(P<0.05),其抑制作用呈剂量依赖关系。结论白藜芦醇具有抑制小鼠CD4+T细胞活化的作用,其抑制作用呈剂量依赖关系。
Objective To study the effect of resveratrol on murine CD4 ＋T lymphocyte activation. Methods Splenic mononuclear cells （SMCs） from specific pathogen free （SPF） BALB/c mice （female, aged 9-10 weeks） were cultured with resveratrol （0, 10, 20, and 40 mmol/L） for 30 rain in vitro, and then were activated with ConA, anti-CD3 or anti-CD3/anti- CD28. At 6 h （for CD69） and 36 h （for CD？I）, cells were harvested for being stained with anti-CD4-PerCP and anti-CD69- FITC, or anti-CD4-PerCP and anti-CD7]-FITC, and the expressions of CD69 and CD7] were detected respectively using flow cytometry. Results Resveratrol （ t〉 l0 mmoVL） significantly decreased CD69 levels on CD4＋ T lymphocytes activated with anti-CD3 in a dose-dependent manner （P 〈0.01）. In addition, resveratrol （ I〉20 mmol/L） decreased CD69 levels on CD4 ＋ T lymphocytes activated with ConA, and anti-CD3/anti-CD28 in a dose-dependent manner （P 〈 0.05）. When concentrations I〉 10 mmoVL, resveratrol depressed CDT] expression on ConA stimulated CD4 ~ T lymphocytes （ P 〈 0.05 ）, and resveratrol （ I〉20 mmoVL） decreased CD71 levels on anti-CD3 and anti-CD3/anti-CD28 stimulated CD4 ＋ T lymphocytes （ P 〈 0.05 ）, all in a dose-dependent manner. Conclusion Resveratrol inhibits the activation of murine CD4 ＋ T lymphocytes in a dose- dependent manner.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2013年第7期677-680,684,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
广州医学院博士科研项目(2012C83)
